In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal

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Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocytes after...

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Autores principales: Sansinena, Marina, Santos, María Victoria, Chirife, Jorge, Zaritzky, Noemí Elisabet
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Exactas
Embryo development
Glass transition
Heat transfer
Oocyte
Vitrification
Warming
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/101441
https://ri.conicet.gov.ar/11336/72219
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-101441
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Embryo development
Glass transition
Heat transfer
Oocyte
Vitrification
Warming
spellingShingle Ciencias Exactas
Embryo development
Glass transition
Heat transfer
Oocyte
Vitrification
Warming
Sansinena, Marina
Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí Elisabet
In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
topic_facet Ciencias Exactas
Embryo development
Glass transition
Heat transfer
Oocyte
Vitrification
Warming
description Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocytes after parthenogenetic activation and culture. The viability of oocytes vitrified in four different cryo-devices was assessed. Consistent with modelling predictions, oocytes vitrified using cryo-devices with the highest modelled cooling rates had significantly (P < 0.05) better cleavage and blastocyst formation rates. We then evaluated a two-step sample removal process, in which oocytes were held in nitrogen vapour for 15 s to simulate sample identification during clinical application, before being removed completely and warmed. Oocytes exposed to this procedure showed reduced developmental potential, according to the model, owing to thermodynamic instability and devitrification at relatively low temperatures. These findings suggest that cryo-device selection and handling, including method of removal from nitrogen storage, are critical to survival of vitrified oocytes. Limitations of the study include use of parthenogenetically activated rather than fertilized ova and lack of physical measurement of recrystallization. We suggest mathematical modelling could be used to predict the effect of critical steps in cryopreservation.
format Articulo
Preprint
author Sansinena, Marina
Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí Elisabet
author_facet Sansinena, Marina
Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí Elisabet
author_sort Sansinena, Marina
title In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_short In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_full In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_fullStr In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_full_unstemmed In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_sort in-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/101441
https://ri.conicet.gov.ar/11336/72219
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AT chirifejorge invitrodevelopmentofvitrifiedwarmedbovineoocytesafteractivationmaybepredictedbasedonmathematicalmodellingofcoolingandwarmingratesduringvitrificationstorageandsampleremoval
AT zaritzkynoemielisabet invitrodevelopmentofvitrifiedwarmedbovineoocytesafteractivationmaybepredictedbasedonmathematicalmodellingofcoolingandwarmingratesduringvitrificationstorageandsampleremoval
bdutipo_str Repositorios
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