Recent advances in β-galactosidase and fructosyltransferase immobilization technology
The highly demanding conditions of industrial processes may lower the stability and affect the activity of enzymes used as biocatalysts. Enzyme immobilization emerged as an approach to promote stabilization and easy removal of enzymes for their reusability. The aim of this review is to go through th...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2020
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/101211 |
| Aporte de: |
| id |
I19-R120-10915-101211 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas b-galactosidase immobilization methods fructosyltransferase supports |
| spellingShingle |
Ciencias Exactas b-galactosidase immobilization methods fructosyltransferase supports Ureta, María Micaela Martins, Gonzalo Nuno Figueira, Onofre Pires, Pedro Filipe Castilho, Paula Cristina Gómez-Zavaglia, Andrea Recent advances in β-galactosidase and fructosyltransferase immobilization technology |
| topic_facet |
Ciencias Exactas b-galactosidase immobilization methods fructosyltransferase supports |
| description |
The highly demanding conditions of industrial processes may lower the stability and affect the activity of enzymes used as biocatalysts. Enzyme immobilization emerged as an approach to promote stabilization and easy removal of enzymes for their reusability. The aim of this review is to go through the principal immobilization strategies addressed to achieve optimal industrial processes with special care on those reported for two types of enzymes: β-galactosidases and fructosyltransferases. The main methods used to immobilize these two enzymes are adsorption, entrapment, covalent coupling and cross-linking or aggregation (no support is used), all of them having pros and cons. Regarding the support, it should be cost-effective, assure the reusability and an easy recovery of the enzyme, increasing its stability and durability. The discussion provided showed that the type of enzyme, its origin, its purity, together with the type of immobilization method and the support will affect the performance during the enzymatic synthesis. Enzymes’ immobilization involves interdisciplinary knowledge including enzymology, nanotechnology, molecular dynamics, cellular physiology and process design. The increasing availability of facilities has opened a variety of possibilities to define strategies to optimize the activity and re-usability of β-galactosidases and fructosyltransferases, but there is still great place for innovative developments. |
| format |
Articulo Articulo |
| author |
Ureta, María Micaela Martins, Gonzalo Nuno Figueira, Onofre Pires, Pedro Filipe Castilho, Paula Cristina Gómez-Zavaglia, Andrea |
| author_facet |
Ureta, María Micaela Martins, Gonzalo Nuno Figueira, Onofre Pires, Pedro Filipe Castilho, Paula Cristina Gómez-Zavaglia, Andrea |
| author_sort |
Ureta, María Micaela |
| title |
Recent advances in β-galactosidase and fructosyltransferase immobilization technology |
| title_short |
Recent advances in β-galactosidase and fructosyltransferase immobilization technology |
| title_full |
Recent advances in β-galactosidase and fructosyltransferase immobilization technology |
| title_fullStr |
Recent advances in β-galactosidase and fructosyltransferase immobilization technology |
| title_full_unstemmed |
Recent advances in β-galactosidase and fructosyltransferase immobilization technology |
| title_sort |
recent advances in β-galactosidase and fructosyltransferase immobilization technology |
| publishDate |
2020 |
| url |
http://sedici.unlp.edu.ar/handle/10915/101211 |
| work_keys_str_mv |
AT uretamariamicaela recentadvancesinbgalactosidaseandfructosyltransferaseimmobilizationtechnology AT martinsgonzalonuno recentadvancesinbgalactosidaseandfructosyltransferaseimmobilizationtechnology AT figueiraonofre recentadvancesinbgalactosidaseandfructosyltransferaseimmobilizationtechnology AT pirespedrofilipe recentadvancesinbgalactosidaseandfructosyltransferaseimmobilizationtechnology AT castilhopaulacristina recentadvancesinbgalactosidaseandfructosyltransferaseimmobilizationtechnology AT gomezzavagliaandrea recentadvancesinbgalactosidaseandfructosyltransferaseimmobilizationtechnology |
| bdutipo_str |
Repositorios |
| _version_ |
1764820440797151232 |