Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle

Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to c...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Goszczynski, Daniel Estanislao, Papaleo Mazzucco, Juliana, Ripoli, María Verónica, Villarreal, Edgardo Leopoldo, Rogberg Muñoz, Andrés, Mezzadra, Carlos Alberto, Melucci, Lilia Magdalena, Giovambattista, Guillermo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/100993
https://ri.conicet.gov.ar/11336/48668
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-100993
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp
spellingShingle Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp
Goszczynski, Daniel Estanislao
Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andrés
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
topic_facet Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp
description Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.
format Articulo
Articulo
author Goszczynski, Daniel Estanislao
Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andrés
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
author_facet Goszczynski, Daniel Estanislao
Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andrés
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
author_sort Goszczynski, Daniel Estanislao
title Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_short Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_full Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_fullStr Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_full_unstemmed Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_sort genetic characterisation of pparg, cebpa and rxra, and their influence on meat quality traits in cattle
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/100993
https://ri.conicet.gov.ar/11336/48668
work_keys_str_mv AT goszczynskidanielestanislao geneticcharacterisationofppargcebpaandrxraandtheirinfluenceonmeatqualitytraitsincattle
AT papaleomazzuccojuliana geneticcharacterisationofppargcebpaandrxraandtheirinfluenceonmeatqualitytraitsincattle
AT ripolimariaveronica geneticcharacterisationofppargcebpaandrxraandtheirinfluenceonmeatqualitytraitsincattle
AT villarrealedgardoleopoldo geneticcharacterisationofppargcebpaandrxraandtheirinfluenceonmeatqualitytraitsincattle
AT rogbergmunozandres geneticcharacterisationofppargcebpaandrxraandtheirinfluenceonmeatqualitytraitsincattle
AT mezzadracarlosalberto geneticcharacterisationofppargcebpaandrxraandtheirinfluenceonmeatqualitytraitsincattle
AT meluccililiamagdalena geneticcharacterisationofppargcebpaandrxraandtheirinfluenceonmeatqualitytraitsincattle
AT giovambattistaguillermo geneticcharacterisationofppargcebpaandrxraandtheirinfluenceonmeatqualitytraitsincattle
bdutipo_str Repositorios
_version_ 1764820440388206592

Ejemplares similares