<i>Brucella abortus</i> invasion of osteocytes modulates connexin 43 and integrin expression and induces osteoclastogenesis via receptor activator of NF-κB ligand and tumor necrosis factor alpha secretion

Osteoarticular brucellosis is the most common localization of human active disease. Osteocytes are the most abundant cells of bone. They secrete factors that regulate the differentiation of both osteoblasts and osteoclasts during bone remodeling. The aim of this study is to determine if Brucella abo...

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Autores principales: Pesce Viglietti, Ayelén Ivana, Arriola Benítez, Paula Constanza, Gentilini, Maria Virginia, Velasquez, Lis Noelia, Fossati, Carlos Alberto, Giambartolomei, Guillermo Hernán, Delpino, María Victoria
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Médicas
Osteocyte
Brucella abortus
Connexin-43
Rankl
Tnf-alpha
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/100974
https://ri.conicet.gov.ar/11336/18740
http://iai.asm.org/content/84/1/11.long
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-100974
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Osteocyte
Brucella abortus
Connexin-43
Rankl
Tnf-alpha
spellingShingle Ciencias Médicas
Osteocyte
Brucella abortus
Connexin-43
Rankl
Tnf-alpha
Pesce Viglietti, Ayelén Ivana
Arriola Benítez, Paula Constanza
Gentilini, Maria Virginia
Velasquez, Lis Noelia
Fossati, Carlos Alberto
Giambartolomei, Guillermo Hernán
Delpino, María Victoria
<i>Brucella abortus</i> invasion of osteocytes modulates connexin 43 and integrin expression and induces osteoclastogenesis via receptor activator of NF-κB ligand and tumor necrosis factor alpha secretion
topic_facet Ciencias Médicas
Osteocyte
Brucella abortus
Connexin-43
Rankl
Tnf-alpha
description Osteoarticular brucellosis is the most common localization of human active disease. Osteocytes are the most abundant cells of bone. They secrete factors that regulate the differentiation of both osteoblasts and osteoclasts during bone remodeling. The aim of this study is to determine if Brucella abortus infection modifies osteocyte function. Our results indicate that B. abortus infection induced matrix metalloproteinase 2 (MMP-2), receptor activator for NF-κB ligand (RANKL), proinflammatory cytokines, and keratinocyte chemoattractant (KC) secretion by osteocytes. In addition, supernatants from B. abortus-infected osteocytes induced bone marrow-derived monocytes (BMM) to undergo osteoclastogenesis. Using neutralizing antibodies against tumor necrosis factor alpha (TNF-α) or osteoprotegerin (OPG), RANKL's decoy receptor, we determined that TNF-α and RANKL are involved in osteoclastogenesis induced by supernatants from B. abortus-infected osteocytes. Connexin 43 (Cx43) and the integrins E11/gp38, integrin-α, integrin-β, and CD44 are involved in cell-cell interactions necessary for osteocyte survival. B. abortus infection inhibited the expression of Cx43 but did not modify the expression of integrins. Yet the expression of both Cx43 and integrins was inhibited by supernatants from B. abortus-infected macrophages. B. abortus infection was not capable of inducing osteocyte apoptosis. However, supernatants from B. abortus-infected macrophages induced osteocyte apoptosis in a dose-dependent manner. Taken together, our results indicate that B. abortus infection could alter osteocyte function, contributing to bone damage.
format Articulo
Articulo
author Pesce Viglietti, Ayelén Ivana
Arriola Benítez, Paula Constanza
Gentilini, Maria Virginia
Velasquez, Lis Noelia
Fossati, Carlos Alberto
Giambartolomei, Guillermo Hernán
Delpino, María Victoria
author_facet Pesce Viglietti, Ayelén Ivana
Arriola Benítez, Paula Constanza
Gentilini, Maria Virginia
Velasquez, Lis Noelia
Fossati, Carlos Alberto
Giambartolomei, Guillermo Hernán
Delpino, María Victoria
author_sort Pesce Viglietti, Ayelén Ivana
title <i>Brucella abortus</i> invasion of osteocytes modulates connexin 43 and integrin expression and induces osteoclastogenesis via receptor activator of NF-κB ligand and tumor necrosis factor alpha secretion
title_short <i>Brucella abortus</i> invasion of osteocytes modulates connexin 43 and integrin expression and induces osteoclastogenesis via receptor activator of NF-κB ligand and tumor necrosis factor alpha secretion
title_full <i>Brucella abortus</i> invasion of osteocytes modulates connexin 43 and integrin expression and induces osteoclastogenesis via receptor activator of NF-κB ligand and tumor necrosis factor alpha secretion
title_fullStr <i>Brucella abortus</i> invasion of osteocytes modulates connexin 43 and integrin expression and induces osteoclastogenesis via receptor activator of NF-κB ligand and tumor necrosis factor alpha secretion
title_full_unstemmed <i>Brucella abortus</i> invasion of osteocytes modulates connexin 43 and integrin expression and induces osteoclastogenesis via receptor activator of NF-κB ligand and tumor necrosis factor alpha secretion
title_sort <i>brucella abortus</i> invasion of osteocytes modulates connexin 43 and integrin expression and induces osteoclastogenesis via receptor activator of nf-κb ligand and tumor necrosis factor alpha secretion
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/100974
https://ri.conicet.gov.ar/11336/18740
http://iai.asm.org/content/84/1/11.long
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