High prevalence of clade 8 <i>Escherichia coli</i> O157:H7 isolated from retail meat and butcher shop environment

Escherichia coli O157:H7 is an enteric pathogen associated with food safety threats and with significant morbidity and mortality worldwide. In Argentina, post-enteric hemolytic uremic syndrome (HUS) is endemic, with >70% of cases associated with E. coli O157 infection. To date the biological basi...

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Autores principales: Galli, Lucía, Brusa, Victoria, Singh, Pallavi, Cataldi, Ángel Adrián, Manning, Shannon, Peral García, Pilar, Leotta, Gerardo Aníbal
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Veterinarias
O157:h7
Clade 8
Meat
Environment
Butcher shops
Argentina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/100907
https://ri.conicet.gov.ar/11336/43967
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-100907
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
O157:h7
Clade 8
Meat
Environment
Butcher shops
Argentina
spellingShingle Ciencias Veterinarias
O157:h7
Clade 8
Meat
Environment
Butcher shops
Argentina
Galli, Lucía
Brusa, Victoria
Singh, Pallavi
Cataldi, Ángel Adrián
Manning, Shannon
Peral García, Pilar
Leotta, Gerardo Aníbal
High prevalence of clade 8 <i>Escherichia coli</i> O157:H7 isolated from retail meat and butcher shop environment
topic_facet Ciencias Veterinarias
O157:h7
Clade 8
Meat
Environment
Butcher shops
Argentina
description Escherichia coli O157:H7 is an enteric pathogen associated with food safety threats and with significant morbidity and mortality worldwide. In Argentina, post-enteric hemolytic uremic syndrome (HUS) is endemic, with >70% of cases associated with E. coli O157 infection. To date the biological basis behind the severity among E. coli O157 infections is unknown. However, single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades, of which clade 8 strains are associated with severe disease cases. The aim of this study was to characterize a collection of 20 STEC O157:H7 strains isolated between 2011 and 2013 from ground beef and different environmental samples from butcher shops of Argentina. All strains harbored the eae, ehxA, fliCH7, efa, iha, and toxB genes, with stx2a/stx2c as the predominant genotype (75%). The XbaI-PFGE analysis showed that the E. coli O157 strains had high genetic diversity. Nine strains were grouped in four XbaI-PFGE clusters, whereas 11 strains showed unique XbaI-PFGE patterns. In contrast, the SNP analysis allowed us to separate the strains in two distinct lineages representing clade 8 (70%) and clade 6 (30%). Our results show the molecular characterization of E. coli O157 strains isolated from ground beef and environmental samples from Argentinean butcher shops.
format Articulo
Preprint
author Galli, Lucía
Brusa, Victoria
Singh, Pallavi
Cataldi, Ángel Adrián
Manning, Shannon
Peral García, Pilar
Leotta, Gerardo Aníbal
author_facet Galli, Lucía
Brusa, Victoria
Singh, Pallavi
Cataldi, Ángel Adrián
Manning, Shannon
Peral García, Pilar
Leotta, Gerardo Aníbal
author_sort Galli, Lucía
title High prevalence of clade 8 <i>Escherichia coli</i> O157:H7 isolated from retail meat and butcher shop environment
title_short High prevalence of clade 8 <i>Escherichia coli</i> O157:H7 isolated from retail meat and butcher shop environment
title_full High prevalence of clade 8 <i>Escherichia coli</i> O157:H7 isolated from retail meat and butcher shop environment
title_fullStr High prevalence of clade 8 <i>Escherichia coli</i> O157:H7 isolated from retail meat and butcher shop environment
title_full_unstemmed High prevalence of clade 8 <i>Escherichia coli</i> O157:H7 isolated from retail meat and butcher shop environment
title_sort high prevalence of clade 8 <i>escherichia coli</i> o157:h7 isolated from retail meat and butcher shop environment
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/100907
https://ri.conicet.gov.ar/11336/43967
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