Development of a peptide ELISA for the diagnosis of equine arteritis virus

A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the...

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Autores principales: Metz, Germán Ernesto, Lorenzon, Serena, María Soledad, Corva, Santiago Gerardo, Panei, Carlos Javier, Díaz, Silvina, Maffud Cilli, Eduardo, Echeverría, María Gabriela
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Veterinarias
Synthetic peptides
ELISA
Equine arteritis virus
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/100584
https://ri.conicet.gov.ar/11336/11505
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-100584
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Synthetic peptides
ELISA
Equine arteritis virus
spellingShingle Ciencias Veterinarias
Synthetic peptides
ELISA
Equine arteritis virus
Metz, Germán Ernesto
Lorenzon,
Serena, María Soledad
Corva, Santiago Gerardo
Panei, Carlos Javier
Díaz, Silvina
Maffud Cilli, Eduardo
Echeverría, María Gabriela
Development of a peptide ELISA for the diagnosis of equine arteritis virus
topic_facet Ciencias Veterinarias
Synthetic peptides
ELISA
Equine arteritis virus
description A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations.
format Articulo
Preprint
author Metz, Germán Ernesto
Lorenzon,
Serena, María Soledad
Corva, Santiago Gerardo
Panei, Carlos Javier
Díaz, Silvina
Maffud Cilli, Eduardo
Echeverría, María Gabriela
author_facet Metz, Germán Ernesto
Lorenzon,
Serena, María Soledad
Corva, Santiago Gerardo
Panei, Carlos Javier
Díaz, Silvina
Maffud Cilli, Eduardo
Echeverría, María Gabriela
author_sort Metz, Germán Ernesto
title Development of a peptide ELISA for the diagnosis of equine arteritis virus
title_short Development of a peptide ELISA for the diagnosis of equine arteritis virus
title_full Development of a peptide ELISA for the diagnosis of equine arteritis virus
title_fullStr Development of a peptide ELISA for the diagnosis of equine arteritis virus
title_full_unstemmed Development of a peptide ELISA for the diagnosis of equine arteritis virus
title_sort development of a peptide elisa for the diagnosis of equine arteritis virus
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/100584
https://ri.conicet.gov.ar/11336/11505
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