cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon

Cucumber mosaic virus (CMV) has the broadest host range among plant viruses, causing enormous losses in agriculture. In melon, strains of subgroup II are unable to establish a systemic infection in the near‐isogenic line SC12‐1‐99, which carries the recessive resistance gene cmv1 from the accession...

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Autores principales:	Guiu Aragonés, Cèlia, Sánchez Pina, María Amelia, Díaz Pendón, Juan Antonio, Peña, Eduardo José, Heinlein, Manfred, Martín Hernández, Ana Montserrat
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2016
Materias:	Biología Ciencias Agrarias Cucumber mosaic virus Recessive resistance Cmv1 Phloem entry Systemic infection Virus movement Host factor
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/100510 https://ri.conicet.gov.ar/11336/47889 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12351
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-100510
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Ciencias Agrarias Cucumber mosaic virus Recessive resistance Cmv1 Phloem entry Systemic infection Virus movement Host factor
spellingShingle	Biología Ciencias Agrarias Cucumber mosaic virus Recessive resistance Cmv1 Phloem entry Systemic infection Virus movement Host factor Guiu Aragonés, Cèlia Sánchez Pina, María Amelia Díaz Pendón, Juan Antonio Peña, Eduardo José Heinlein, Manfred Martín Hernández, Ana Montserrat cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
topic_facet	Biología Ciencias Agrarias Cucumber mosaic virus Recessive resistance Cmv1 Phloem entry Systemic infection Virus movement Host factor
description	Cucumber mosaic virus (CMV) has the broadest host range among plant viruses, causing enormous losses in agriculture. In melon, strains of subgroup II are unable to establish a systemic infection in the near‐isogenic line SC12‐1‐99, which carries the recessive resistance gene cmv1 from the accession PI 161375, cultivar ‘Songwhan Charmi’. Strains of subgroup I overcome cmv1 resistance in a manner dependent on the movement protein. We characterized the resistance conferred by cmv1 and established that CMV‐LS (subgroup II) can move from cell to cell up to the veins in the inoculated leaf, but cannot enter the phloem. Immunogold labelling at transmission electron microscopy level showed that CMV‐LS remains restricted to the bundle sheath (BS) cells in the resistant line, and does not invade vascular parenchyma or intermediary cells, whereas, in the susceptible line ‘Piel de Sapo’ (PS), the virus invades all vein cell types. These observations indicate that the resistant allele of cmv1 restricts systemic infection in a virus strain‐ and cell type‐specific manner by acting as an important gatekeeper for virus progression from BS cells to phloem cells. Graft inoculation experiments showed that CMV‐LS cannot move from the infected PS stock into the resistant cmv1 scion, thus suggesting an additional role for cmv1 related to CMV transport within or exit from the phloem. The characterization of this new form of recessive resistance, based on a restriction of virus systemic movement, opens up the possibility to design alternative approaches for breeding strategies in melon.
format	Articulo Articulo
author	Guiu Aragonés, Cèlia Sánchez Pina, María Amelia Díaz Pendón, Juan Antonio Peña, Eduardo José Heinlein, Manfred Martín Hernández, Ana Montserrat
author_facet	Guiu Aragonés, Cèlia Sánchez Pina, María Amelia Díaz Pendón, Juan Antonio Peña, Eduardo José Heinlein, Manfred Martín Hernández, Ana Montserrat
author_sort	Guiu Aragonés, Cèlia
title	cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_short	cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_full	cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_fullStr	cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_full_unstemmed	cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_sort	cmv1 is a gate for cucumber mosaic virus transport from bundle sheath cells to phloem in melon
publishDate	2016
url	http://sedici.unlp.edu.ar/handle/10915/100510 https://ri.conicet.gov.ar/11336/47889 https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12351
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bdutipo_str	Repositorios
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cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon

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