Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols

Cryopreservation protocols include the use of additives and pre-treatments aimed to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between the equilibrium freezing (Tf)...

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Autores principales: Teixeira, Aline S., Gonzalez Benito, M. Elena, Molina García, Antonio D.
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2014
Materias:
Química
Cooling
Warming
Rates
Vitrification
Droplet-vitrification
Plant
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/100147
https://ri.conicet.gov.ar/11336/10655
http://onlinelibrary.wiley.com/doi/10.1002/btpr.1938/abstract
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-100147
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Cooling
Warming
Rates
Vitrification
Droplet-vitrification
Plant
spellingShingle Química
Cooling
Warming
Rates
Vitrification
Droplet-vitrification
Plant
Teixeira, Aline S.
Gonzalez Benito, M. Elena
Molina García, Antonio D.
Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
topic_facet Química
Cooling
Warming
Rates
Vitrification
Droplet-vitrification
Plant
description Cryopreservation protocols include the use of additives and pre-treatments aimed to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between the equilibrium freezing (Tf) and the glass transition (TG) temperatures. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage. Vitrification and droplet-vitrification techniques, frequently used cryopreservation protocols based in fast cooling, were studied, alongside with the corresponding warming procedures. A very fast data acquisition system, able to read very low temperatures, down to that of liquid nitrogen, was employed. Cooling rates, measured between -20 and -120 ºC, ranged from ca. 5 ºC s-1 to 400 ºC s-1, while warming rates spanned from ca. 2 ºC s-1 to 280 ºC s-1, for the different protocols and conditions studied. A wider measuring window (0 ºC to -150 ºC) produced lower rates for all cases. The cooling and warming rates were also related to the survival observed after the different procedures. Those protocols with the faster rates yielded the highest survival percentages.
format Articulo
Preprint
author Teixeira, Aline S.
Gonzalez Benito, M. Elena
Molina García, Antonio D.
author_facet Teixeira, Aline S.
Gonzalez Benito, M. Elena
Molina García, Antonio D.
author_sort Teixeira, Aline S.
title Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_short Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_full Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_fullStr Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_full_unstemmed Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_sort measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/100147
https://ri.conicet.gov.ar/11336/10655
http://onlinelibrary.wiley.com/doi/10.1002/btpr.1938/abstract
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bdutipo_str Repositorios
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