Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation
The potential of important agro-industrial wastes, apple pomace (AP) and orange peel (OP) as C sources, was investigated in the maximization of polygalacturonase (PG), an industrially significant enzyme, using an industrially important microorganism Aspergillus sojae. Factors such as various hydroly...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | Articulo Preprint |
| Lenguaje: | Inglés |
| Publicado: |
2015
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/100087 https://ri.conicet.gov.ar/11336/16074 http://www.tandfonline.com/doi/full/10.1080/09593330.2015.1042922 |
| Aporte de: |
| id |
I19-R120-10915-100087 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Química Agro-industrial waste Polygalacturonase Apple pomace Orange peel Aspergillus sojae |
| spellingShingle |
Química Agro-industrial waste Polygalacturonase Apple pomace Orange peel Aspergillus sojae Göğüş, Nihan Evcan, Ezgi Tari, Canan Cavalitto, Sebastián Fernando Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation |
| topic_facet |
Química Agro-industrial waste Polygalacturonase Apple pomace Orange peel Aspergillus sojae |
| description |
The potential of important agro-industrial wastes, apple pomace (AP) and orange peel (OP) as C sources, was investigated in the maximization of polygalacturonase (PG), an industrially significant enzyme, using an industrially important microorganism Aspergillus sojae. Factors such as various hydrolysis forms of the C sources (hydrolysed-AP, non-hydrolysed-AP, hydrolysed-AP + OP, non-hydrolysed-AP + OP) and N sources (ammonium sulphate and urea), and incubation time (4, 6, and 8 days) were screened. It was observed that maximum PG activity was achieved at a combination of non-hydrolysed-AP + OP and ammonium sulphate with eight days of incubation. For the pre-optimization study, ammonium sulphate concentration and the mixing ratios of AP + OP at different total C concentrations (9, 15, 21 g l−1) were evaluated. The optimum conditions for the maximum PG production (144.96 U ml−1) was found as 21 g l−1 total carbohydrate concentration totally coming from OP at 15 g l−1 ammonium sulphate concentration. On the other hand, 3:1 mixing ratio of OP + AP at 11.50 g l−1 ammonium sulphate concentration also resulted in a considerable PG activity (115.73 U ml−1). These results demonstrated that AP can be evaluated as an additional C source to OP for PG production, which in turn both can be alternative solutions for the elimination of the waste accumulation in the food industry with economical returns. |
| format |
Articulo Preprint |
| author |
Göğüş, Nihan Evcan, Ezgi Tari, Canan Cavalitto, Sebastián Fernando |
| author_facet |
Göğüş, Nihan Evcan, Ezgi Tari, Canan Cavalitto, Sebastián Fernando |
| author_sort |
Göğüş, Nihan |
| title |
Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation |
| title_short |
Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation |
| title_full |
Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation |
| title_fullStr |
Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation |
| title_full_unstemmed |
Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation |
| title_sort |
evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation |
| publishDate |
2015 |
| url |
http://sedici.unlp.edu.ar/handle/10915/100087 https://ri.conicet.gov.ar/11336/16074 http://www.tandfonline.com/doi/full/10.1080/09593330.2015.1042922 |
| work_keys_str_mv |
AT gogusnihan evaluationofagroindustrialwastestheirstateandmixingratioformaximumpolygalacturonaseandbiomassproductioninsubmergedfermentation AT evcanezgi evaluationofagroindustrialwastestheirstateandmixingratioformaximumpolygalacturonaseandbiomassproductioninsubmergedfermentation AT taricanan evaluationofagroindustrialwastestheirstateandmixingratioformaximumpolygalacturonaseandbiomassproductioninsubmergedfermentation AT cavalittosebastianfernando evaluationofagroindustrialwastestheirstateandmixingratioformaximumpolygalacturonaseandbiomassproductioninsubmergedfermentation |
| bdutipo_str |
Repositorios |
| _version_ |
1764820439824072704 |