Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples

The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed...

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Autores principales: Krüger, Alejandra, Lucchesi, Paula Maria Alejandra, Sanso, Andrea Mariel, Etcheverría, Analía Inés, Bustamante, Ana Victoria, Burgán, Julia, Fernández, Luciana, Fernández, Daniel, Leotta, Gerardo Aníbal, Friedrich, Alexander W., Padola, Nora Lía, Rossen, John W. A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/100009
https://ri.conicet.gov.ar/11336/11804
http://journal.frontiersin.org/article/10.3389/fcimb.2015.00074/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/
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SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-100009
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle
spellingShingle Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle
Krüger, Alejandra
Lucchesi, Paula Maria Alejandra
Sanso, Andrea Mariel
Etcheverría, Analía Inés
Bustamante, Ana Victoria
Burgán, Julia
Fernández, Luciana
Fernández, Daniel
Leotta, Gerardo Aníbal
Friedrich, Alexander W.
Padola, Nora Lía
Rossen, John W. A.
Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
topic_facet Ciencias Veterinarias
Shiga toxin-producing Escherichia coli
O26:H11
Genetic characterization
Microarray
MLVA
Hemolytic uremic syndrome
Cattle
description The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a.
format Articulo
Articulo
author Krüger, Alejandra
Lucchesi, Paula Maria Alejandra
Sanso, Andrea Mariel
Etcheverría, Analía Inés
Bustamante, Ana Victoria
Burgán, Julia
Fernández, Luciana
Fernández, Daniel
Leotta, Gerardo Aníbal
Friedrich, Alexander W.
Padola, Nora Lía
Rossen, John W. A.
author_facet Krüger, Alejandra
Lucchesi, Paula Maria Alejandra
Sanso, Andrea Mariel
Etcheverría, Analía Inés
Bustamante, Ana Victoria
Burgán, Julia
Fernández, Luciana
Fernández, Daniel
Leotta, Gerardo Aníbal
Friedrich, Alexander W.
Padola, Nora Lía
Rossen, John W. A.
author_sort Krüger, Alejandra
title Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_short Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_full Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_fullStr Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_full_unstemmed Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_sort genetic characterization of shiga toxin-producing escherichia coli o26:h11 strains isolated from animal, food, and clinical samples
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/100009
https://ri.conicet.gov.ar/11336/11804
http://journal.frontiersin.org/article/10.3389/fcimb.2015.00074/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/
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