In vitro effect of methotrexate on erythrocytes rheological variables.

Methotrexate is one of the drugs that is generally used in cancer and rheumatoid arthritis treatments. Anemia is one of the adverse reactions found; for which we decided to study the direct effect of methotrexate on erythrocyte rheology in vitro, using normal blood donor samples that were divided in...

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Autores principales: Martino, Matias, Urli, Leda, Svetaz, Maria José, Georgetti, María Edith, Volpintesta, Ricardo, Luquita, Alejandra
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional de Rosario 2022
Materias:
Metotrexato
Artritis Reumatoide
Anemia
Reología eritrocitaria
Methotrexate
Rheumatoid arthritis
Red cell rheology
Acceso en línea:https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/63
Aporte de:
FCM Científica de Universidad Nacional de Rosario
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institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-235
container_title_str FCM Científica
language Español
format Artículo revista
topic Metotrexato
Artritis Reumatoide
Anemia
Reología eritrocitaria
Methotrexate
Rheumatoid arthritis
Anemia
Red cell rheology
spellingShingle Metotrexato
Artritis Reumatoide
Anemia
Reología eritrocitaria
Methotrexate
Rheumatoid arthritis
Anemia
Red cell rheology
Martino, Matias
Urli, Leda
Svetaz, Maria José
Georgetti, María Edith
Volpintesta, Ricardo
Luquita, Alejandra
In vitro effect of methotrexate on erythrocytes rheological variables.
topic_facet Metotrexato
Artritis Reumatoide
Anemia
Reología eritrocitaria
Methotrexate
Rheumatoid arthritis
Anemia
Red cell rheology
author Martino, Matias
Urli, Leda
Svetaz, Maria José
Georgetti, María Edith
Volpintesta, Ricardo
Luquita, Alejandra
author_facet Martino, Matias
Urli, Leda
Svetaz, Maria José
Georgetti, María Edith
Volpintesta, Ricardo
Luquita, Alejandra
author_sort Martino, Matias
title In vitro effect of methotrexate on erythrocytes rheological variables.
title_short In vitro effect of methotrexate on erythrocytes rheological variables.
title_full In vitro effect of methotrexate on erythrocytes rheological variables.
title_fullStr In vitro effect of methotrexate on erythrocytes rheological variables.
title_full_unstemmed In vitro effect of methotrexate on erythrocytes rheological variables.
title_sort in vitro effect of methotrexate on erythrocytes rheological variables.
description Methotrexate is one of the drugs that is generally used in cancer and rheumatoid arthritis treatments. Anemia is one of the adverse reactions found; for which we decided to study the direct effect of methotrexate on erythrocyte rheology in vitro, using normal blood donor samples that were divided in 5 aliquots: one was control and the other were treated , with methotrexat in increasing concentrations. (n: 5, each group). Cell morphology was estimated by light microscope; Rigidity index by filtration; Osmotic fragility (measured photometrically at 540 nm), obtaining X50 value (NaCl mM concentration yielding 50% hemolysis) and β (degree of homogeneity of the cell population). Erythrocyte aggregation was measured by optical densitometry showing S, mean size of red cells aggregates and V, initial velocity of process. There was no alteration of erythrocyte aggregation neither in S nor in V. Presence of methotrexat in incubation medium increased the osmotic fragility (higher X50) as well as heterogeneity of the population response to hyposmolarity in the 1 and 5 µM doses. The former effects added to stomatocytosis and erythrocyte deformability could be related with the anemia in patients under Methotrexat treatment. In erythrocyte aggregation, although the size and speed of the aggregates do not increase in presence of the drug, it would be expected that when the rigid erythrocytes enters the capillaries could give time to the formation of aggregates that difficult circulation. and promote the destruction of red blood cells.
publisher Universidad Nacional de Rosario
publishDate 2022
url https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/63
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first_indexed 2023-06-26T22:51:02Z
last_indexed 2023-06-26T22:51:02Z
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spelling I15-R235-article-632022-07-28T12:03:42Z In vitro effect of methotrexate on erythrocytes rheological variables. Efecto in vitro del metotrexato sobre las variables reológicas de los eritrocitos. Martino, Matias Urli, Leda Svetaz, Maria José Georgetti, María Edith Volpintesta, Ricardo Luquita, Alejandra Metotrexato Artritis Reumatoide Anemia Reología eritrocitaria Methotrexate Rheumatoid arthritis Anemia Red cell rheology Methotrexate is one of the drugs that is generally used in cancer and rheumatoid arthritis treatments. Anemia is one of the adverse reactions found; for which we decided to study the direct effect of methotrexate on erythrocyte rheology in vitro, using normal blood donor samples that were divided in 5 aliquots: one was control and the other were treated , with methotrexat in increasing concentrations. (n: 5, each group). Cell morphology was estimated by light microscope; Rigidity index by filtration; Osmotic fragility (measured photometrically at 540 nm), obtaining X50 value (NaCl mM concentration yielding 50% hemolysis) and β (degree of homogeneity of the cell population). Erythrocyte aggregation was measured by optical densitometry showing S, mean size of red cells aggregates and V, initial velocity of process. There was no alteration of erythrocyte aggregation neither in S nor in V. Presence of methotrexat in incubation medium increased the osmotic fragility (higher X50) as well as heterogeneity of the population response to hyposmolarity in the 1 and 5 µM doses. The former effects added to stomatocytosis and erythrocyte deformability could be related with the anemia in patients under Methotrexat treatment. In erythrocyte aggregation, although the size and speed of the aggregates do not increase in presence of the drug, it would be expected that when the rigid erythrocytes enters the capillaries could give time to the formation of aggregates that difficult circulation. and promote the destruction of red blood cells. El metotrexato es uno de los fármacos que generalmente se emplea en el tratamiento del cáncer y de la artritis reumatoide. Entre las reacciones adversas encontradas está la anemia, por lo que nos propusimos estudiar el efecto directo del metotrexato sobre la reología eritrocitaria in vitro, utilizando muestras de donantes de sangre normales que se dividieron en 5 alícuotas: una se utilizó como control y a las otras se les adicionó metotrexato en concentraciones crecientes (n:5, cada grupo). La forma celular fue estimada con un microscopio óptico; el índice de rigidez por filtración; la fragilidad osmótica (medida fotométricamente a 540 nm), obteniendo valor X50 (concentración de NaCl mM con 50% de hemólisis) y β (grado de homogeneidad de la población celular). La agregación eritrocitaria medida por densitometría óptica ha estimado S, tamaño promedio de los agregados eritrocitarios y V, velocidad inicial del proceso. La presencia de metotrexato en el medio de incubación aumentó la fragilidad osmótica (mayor X50) e incrementó la heterogeneidad de la respuesta poblacional a la hiposmolaridad con las dosis de 1 y 5 µM. Estos efectos sumados a la estomatocitosis y menor deformabilidad pueden relacionarse con la anemia encontrada en los pacientes tratados con metotrexato. En la agregación eritrocitaria, si bien el tamaño y la velocidad de los agregados no se incrementan en presencia del fármaco, sería esperable que la dificultad de los eritrocitos con rigidez al ingresar en los capilares pueda dar tiempo a la formación de agregados que dificulten la circulación y favorezcan la destrucción de los glóbulos rojos. Universidad Nacional de Rosario 2022-07-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/63 10.35305/fcm.v2i.63 Revista de la Facultad de Ciencias Médicas. Universidad Nacional de Rosario.; Vol. 2 (2021): Revista de la Facultad de Ciencias Médicas. Universidad Nacional de Rosario.; 62-64 2796-7719 spa https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/63/72 Derechos de autor 2022 Revista de la Facultad de Ciencias Médicas. Universidad Nacional de Rosario. https://creativecommons.org/licenses/by-nc/4.0

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