“STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”
Introduction: The infusion of the crude extract Ligaria cuneifolia (Lc host Chañar), is used in folk medicine for its supposed antihypertensive action (1,2,3). Objective: To investigate the potential vasoactive effect of proanthocyanidin-enriched extract extracted from Lc (PLc) on isolated rats aort...
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| Autores principales: | , , , , , |
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| Formato: | Artículo revista |
| Lenguaje: | Español |
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Universidad Nacional de Rosario
2025
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| Acceso en línea: | https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/114 |
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| Sumario: | Introduction: The infusion of the crude extract Ligaria cuneifolia (Lc host Chañar), is used in folk medicine for its supposed antihypertensive action (1,2,3).
Objective: To investigate the potential vasoactive effect of proanthocyanidin-enriched extract extracted from Lc (PLc) on isolated rats aortic rings (4,5).
Materials and Methods: aorta rings were placed in a 20 mL chamber with modified Krebs-Henseleit solution (KHM) balancing out for 60 min. Isometric vessel tension was measured with a force displacement transducer connected to a polygraph. Subsequently, the rings were contracted with 70 mM K+, repeating the procedure until a stable maximal contraction was achieved; post-stabilization for 10 min, vessels were contracted with phenylephrine (0.1µM) and, upon reaching a stable maximum contraction, PLc was added at increasing concentrations (7.14 x 10-6; 7.14 x 10-5; 7.14 x 10-4; 7.14 x 10-3; 7.14 x 10-2; 7.14 x 10-1; and 71.4 µg/mL) so as to create a concentration-response curve. Then, the vessels were again contracted with K+ and finally contracted with for the acetylcholine test (ACh; 1 µM).
Results: The concentration of PLc that induced maximal relaxation was 71.4 µg/mL. The PLc concentration producing 50% relaxation of 6.86 µg/mL (n=4) was estimated using a 4-parameter logistic equation (R2 = 0.92).
Discussion: PLc has an endothelium-dependent vasodilator effect, the mechanical removal of the endothelium abolishes the relaxant activity of PLc. It reached a magnitude equivalent to the maximal ACh-induced response of about 80% of basal tension.
Conclusion: Results support the popular use of Lc. as a hypotensive.
Acknowledgment: Rosario National University
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