“STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”

Introduction: The infusion of the crude extract Ligaria cuneifolia (Lc host Chañar), is used in folk medicine for its supposed antihypertensive action (1,2,3). Objective: To investigate the potential vasoactive effect of proanthocyanidin-enriched extract extracted from Lc (PLc) on isolated rats aort...

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Autores principales:	Gerschcovsky, Natasha, Knox, Marcela, Carnovale, Cristina, Wagner, Marcelo, Vinet, Raul, Luquita, Alejandra
Formato:	Artículo revista
Lenguaje:	Español
Publicado:	Universidad Nacional de Rosario 2025
Materias:	Ligaria cuneifolia proantocianidinas vasodilatación Ligaria Cuneifolia; Proanthocyanidins; Hypotensive; Vasodilatation; Hypotensor
Acceso en línea:	https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/114
Aporte de:	FCM Científica de Universidad Nacional de Rosario

id	I15-R235-article-114
record_format	ojs
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-235
container_title_str	FCM Científica
language	Español
format	Artículo revista
topic	Ligaria cuneifolia proantocianidinas vasodilatación Ligaria Cuneifolia; Proanthocyanidins; Hypotensive; Vasodilatation; Hypotensor
spellingShingle	Ligaria cuneifolia proantocianidinas vasodilatación Ligaria Cuneifolia; Proanthocyanidins; Hypotensive; Vasodilatation; Hypotensor Gerschcovsky, Natasha Knox, Marcela Carnovale, Cristina Wagner, Marcelo Vinet, Raul Luquita, Alejandra “STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”
topic_facet	Ligaria cuneifolia proantocianidinas vasodilatación Ligaria Cuneifolia; Proanthocyanidins; Hypotensive; Vasodilatation; Hypotensor
author	Gerschcovsky, Natasha Knox, Marcela Carnovale, Cristina Wagner, Marcelo Vinet, Raul Luquita, Alejandra
author_facet	Gerschcovsky, Natasha Knox, Marcela Carnovale, Cristina Wagner, Marcelo Vinet, Raul Luquita, Alejandra
author_sort	Gerschcovsky, Natasha
title	“STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”
title_short	“STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”
title_full	“STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”
title_fullStr	“STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”
title_full_unstemmed	“STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”
title_sort	“study of the vasodilatory effect of proanthocyanidins extracted from ligaria cuneifolia (plc)”: “proanthocyanidins extracted from ligaria cuneifolia (plc)”
description	Introduction: The infusion of the crude extract Ligaria cuneifolia (Lc host Chañar), is used in folk medicine for its supposed antihypertensive action (1,2,3). Objective: To investigate the potential vasoactive effect of proanthocyanidin-enriched extract extracted from Lc (PLc) on isolated rats aortic rings (4,5). Materials and Methods: aorta rings were placed in a 20 mL chamber with modified Krebs-Henseleit solution (KHM) balancing out for 60 min. Isometric vessel tension was measured with a force displacement transducer connected to a polygraph. Subsequently, the rings were contracted with 70 mM K+, repeating the procedure until a stable maximal contraction was achieved; post-stabilization for 10 min, vessels were contracted with phenylephrine (0.1µM) and, upon reaching a stable maximum contraction, PLc was added at increasing concentrations (7.14 x 10-6; 7.14 x 10-5; 7.14 x 10-4; 7.14 x 10-3; 7.14 x 10-2; 7.14 x 10-1; and 71.4 µg/mL) so as to create a concentration-response curve. Then, the vessels were again contracted with K+ and finally contracted with for the acetylcholine test (ACh; 1 µM). Results: The concentration of PLc that induced maximal relaxation was 71.4 µg/mL. The PLc concentration producing 50% relaxation of 6.86 µg/mL (n=4) was estimated using a 4-parameter logistic equation (R2 = 0.92). Discussion: PLc has an endothelium-dependent vasodilator effect, the mechanical removal of the endothelium abolishes the relaxant activity of PLc. It reached a magnitude equivalent to the maximal ACh-induced response of about 80% of basal tension. Conclusion: Results support the popular use of Lc. as a hypotensive. Acknowledgment:  Rosario National University
publisher	Universidad Nacional de Rosario
publishDate	2025
url	https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/114
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spelling	I15-R235-article-1142025-07-04T18:04:41Z “STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)” Estudio del efecto vasodilatador de proantocianidinas extraídas de Ligaria cuneifolia: Proantocianidinas extraídas de Ligaria cuneifolia (PLc) Gerschcovsky, Natasha Knox, Marcela Carnovale, Cristina Wagner, Marcelo Vinet, Raul Luquita, Alejandra Ligaria cuneifolia proantocianidinas vasodilatación Ligaria Cuneifolia; Proanthocyanidins; Hypotensive; Vasodilatation; Hypotensor Introduction: The infusion of the crude extract Ligaria cuneifolia (Lc host Chañar), is used in folk medicine for its supposed antihypertensive action (1,2,3). Objective: To investigate the potential vasoactive effect of proanthocyanidin-enriched extract extracted from Lc (PLc) on isolated rats aortic rings (4,5). Materials and Methods: aorta rings were placed in a 20 mL chamber with modified Krebs-Henseleit solution (KHM) balancing out for 60 min. Isometric vessel tension was measured with a force displacement transducer connected to a polygraph. Subsequently, the rings were contracted with 70 mM K+, repeating the procedure until a stable maximal contraction was achieved; post-stabilization for 10 min, vessels were contracted with phenylephrine (0.1µM) and, upon reaching a stable maximum contraction, PLc was added at increasing concentrations (7.14 x 10-6; 7.14 x 10-5; 7.14 x 10-4; 7.14 x 10-3; 7.14 x 10-2; 7.14 x 10-1; and 71.4 µg/mL) so as to create a concentration-response curve. Then, the vessels were again contracted with K+ and finally contracted with for the acetylcholine test (ACh; 1 µM). Results: The concentration of PLc that induced maximal relaxation was 71.4 µg/mL. The PLc concentration producing 50% relaxation of 6.86 µg/mL (n=4) was estimated using a 4-parameter logistic equation (R2 = 0.92). Discussion: PLc has an endothelium-dependent vasodilator effect, the mechanical removal of the endothelium abolishes the relaxant activity of PLc. It reached a magnitude equivalent to the maximal ACh-induced response of about 80% of basal tension. Conclusion: Results support the popular use of Lc. as a hypotensive. Acknowledgment:  Rosario National University   Introducción: La infusión del extracto crudo Ligaria cuneifolia (Lc hospedante Chañar) se utiliza en medicina popular por una supuesta acción antihipertensiva(1,2,3). Objetivo: Investigar el potencial efecto vasoactivo PLc sobre anillos de aorta aislada de ratas(4,5). Materiales y métodos: Los anillos fueron colocados en una cámara de 20 mL con solución de Krebs-Henseleit modificada, equilibrándose durante 60 min. Medimos la tensión isométrica de los vasos con un transductor de desplazamiento de fuerza conectado a un polígrafo. Posteriormente, los anillos fueron contraídos con K+ 70 mM, repitiendo el procedimiento hasta conseguir una contracción máxima estable; post-estabilización de 10 min., los vasos fueron contraídos con fenilefrina (Phe; 0,1µM) y, al alcanzar una contracción máxima estable, se agregó PLc en concentraciones crecientes (7,14 x 10-6; 7,14 x 10-5; 7,14 x 10-4; 7,14 x 10-3; 7,14 x 10-2; 7,14 x 10-1; y 71,4 µg/mL) construyendo una curva concentración-respuesta. Luego, los vasos fueron contraídos nuevamente con K+ y finalmente con Phe para el ensayo de acetilcolina (ACh; 1 µM). Resultados: La concentración de PLc que indujo la relajación máxima fue 71,4 µg/mL. La que produce un 50% de relajación (EC50) de 6,86 µg/mL (n=4) fue estimada a través de una ecuación logística de 4 parámetros (R2 = 0.92). Discusión: PLc posee efecto vasodilatador dependiente de endotelio, su remoción mecánica anula esta actividad. El efecto alcanzó una magnitud equivalente a la respuesta máxima inducida por ACh, de un 80% respecto a la tensión basal Conclusión: Esto apoyaría el uso popular como hipotensor. Agradecimientos: Universidad Nacional de Rosario. Universidad Nacional de Rosario 2025-07-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/114 10.35305/fcm.v4i.114 Revista de la Facultad de Ciencias Médicas. Universidad Nacional de Rosario.; Vol. 4 (2025) 2796-7719 spa https://fcmcientifica.unr.edu.ar/index.php/revista/article/view/114/108 Derechos de autor 2025 Revista de la Facultad de Ciencias Médicas. Universidad Nacional de Rosario. https://creativecommons.org/licenses/by-nc/4.0

“STUDY OF THE VASODILATORY EFFECT OF PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”: “PROANTHOCYANIDINS EXTRACTED FROM LIGARIA CUNEIFOLIA (PLc)”

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