Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

A new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation–emission fluorescence matrix (EEFM) data in standard addition mode and second–order chemometric analysis. The method is simple, fast and eco–friendly because it comp...

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Detalles Bibliográficos
Autores principales: Lozano, Valeria Antonella, Privitera, María Laura
Lenguaje:Inglés
Publicado: Elsevier 2017
Materias:
Human urine samples
Green analytical chemistry
Second–order standard addition
Fluorescence
Chemometrics
Riboflavin
Acceso en línea:http://hdl.handle.net/2133/9737
http://hdl.handle.net/2133/9737
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-9737
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Human urine samples
Green analytical chemistry
Second–order standard addition
Fluorescence
Chemometrics
Riboflavin
spellingShingle Human urine samples
Green analytical chemistry
Second–order standard addition
Fluorescence
Chemometrics
Riboflavin
Lozano, Valeria Antonella
Privitera, María Laura
Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps
topic_facet Human urine samples
Green analytical chemistry
Second–order standard addition
Fluorescence
Chemometrics
Riboflavin
description A new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation–emission fluorescence matrix (EEFM) data in standard addition mode and second–order chemometric analysis. The method is simple, fast and eco–friendly because it complies with the green analytical chemistry principles, avoiding the need of previous clean up and separation steps that consume high amounts of organic solvents. Successful results were obtained by different chemometric algorithms, namely parallel factor analysis (PARAFAC), unfolded partial least–squares/residual bilinearization (U–PLS/RBL) and multidimensional partial least–squares/residual bilinearization (N–PLS/RBL), all in the modified standard addition. These algorithms allowed us to achieve selectivity in a system, which requires standard addition and shows a significant background spectral overlapping with the studied vitamin. The quality of the proposed strategy was evidenced on the basis of the analytical recoveries from urine samples spiked with RF. The detection limits achieved in urine samples are encouraging compared to those obtained using chromatographic approaches. The relative prediction errors were lower than 5.6 %.
author Lozano, Valeria Antonella
Privitera, María Laura
author_facet Lozano, Valeria Antonella
Privitera, María Laura
author_sort Lozano, Valeria Antonella
title Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps
title_short Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps
title_full Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps
title_fullStr Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps
title_full_unstemmed Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps
title_sort development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps
publisher Elsevier
publishDate 2017
url http://hdl.handle.net/2133/9737
http://hdl.handle.net/2133/9737
work_keys_str_mv AT lozanovaleriaantonella developmentofasecondorderstandardadditionfluorescencemethodforthedirectdeterminationofriboflavininhumanurinesampleswithoutpreviouscleanupandseparationsteps
AT priviteramarialaura developmentofasecondorderstandardadditionfluorescencemethodforthedirectdeterminationofriboflavininhumanurinesampleswithoutpreviouscleanupandseparationsteps
bdutipo_str Repositorios
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