Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity

Immunological methods involving pregnancy associated glycoproteins (PAG) are used for cattle pregnancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation o...

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Detalles Bibliográficos
Autores principales: Ruiz Álvarez, Jimena Inés, Teijeiro, Juan Manuel, Marini, Patricia Estela
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: SciRP 2017
Materias:
Anticuerpo
Péptido
Bovino
Preñez
Antibodies
Pregnancy
Cattle
Acceso en línea:http://hdl.handle.net/2133/9731
http://hdl.handle.net/2133/9731
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
Descripción
Sumario:Immunological methods involving pregnancy associated glycoproteins (PAG) are used for cattle pregnancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation of antibodies specific for peptides is a method of choice. In this work, we summarize a series of considerations regarding peptide design and choose free access NCBI, Antigenicity Plot, EMBOSS Antigenic and Expasy tools to apply them. We design peptides specific for different reported PAG members and obtain the corresponding polyclonal antibodies for five of them.

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