Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity

Immunological methods involving pregnancy associated glycoproteins (PAG) are used for cattle pregnancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation o...

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Detalles Bibliográficos
Autores principales: Ruiz Álvarez, Jimena Inés, Teijeiro, Juan Manuel, Marini, Patricia Estela
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: SciRP 2017
Materias:
Anticuerpo
Péptido
Bovino
Preñez
Antibodies
Pregnancy
Cattle
Acceso en línea:http://hdl.handle.net/2133/9731
http://hdl.handle.net/2133/9731
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-9731
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Anticuerpo
Péptido
Bovino
Preñez
Antibodies
Pregnancy
Cattle
spellingShingle Anticuerpo
Péptido
Bovino
Preñez
Antibodies
Pregnancy
Cattle
Ruiz Álvarez, Jimena Inés
Teijeiro, Juan Manuel
Marini, Patricia Estela
Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
topic_facet Anticuerpo
Péptido
Bovino
Preñez
Antibodies
Pregnancy
Cattle
description Immunological methods involving pregnancy associated glycoproteins (PAG) are used for cattle pregnancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation of antibodies specific for peptides is a method of choice. In this work, we summarize a series of considerations regarding peptide design and choose free access NCBI, Antigenicity Plot, EMBOSS Antigenic and Expasy tools to apply them. We design peptides specific for different reported PAG members and obtain the corresponding polyclonal antibodies for five of them.
format article
artículo
publishedVersion
author Ruiz Álvarez, Jimena Inés
Teijeiro, Juan Manuel
Marini, Patricia Estela
author_facet Ruiz Álvarez, Jimena Inés
Teijeiro, Juan Manuel
Marini, Patricia Estela
author_sort Ruiz Álvarez, Jimena Inés
title Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
title_short Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
title_full Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
title_fullStr Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
title_full_unstemmed Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
title_sort preparation of monospecific anti-pag antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
publisher SciRP
publishDate 2017
url http://hdl.handle.net/2133/9731
http://hdl.handle.net/2133/9731
work_keys_str_mv AT ruizalvarezjimenaines preparationofmonospecificantipagantibodiesforcattlepregnancydetectionuseofsyntheticpeptidestoimprovespecificity
AT teijeirojuanmanuel preparationofmonospecificantipagantibodiesforcattlepregnancydetectionuseofsyntheticpeptidestoimprovespecificity
AT marinipatriciaestela preparationofmonospecificantipagantibodiesforcattlepregnancydetectionuseofsyntheticpeptidestoimprovespecificity
bdutipo_str Repositorios
_version_ 1764820413989257217

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