Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.

HPV204 is the only newly identified Mupapillomavirus (Mu-PV) type in more than a decade. To comprehensively characterize HPV204, we performed a detailed molecular analysis of the viral genome and evaluated its clinical relevance in comparison to the other Mu-PVs, HPV1 and HPV63. The 7,227-bp long ge...

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Autores principales:	Chouhy, Diego, Bolatti, Elisa María, Seme, Katja, Poljak, Mario, Giri, Adriana Angélica, Šterbenc, Anja, Hošnjak, Lea, Oštrbenk, Anja, Kocjan, Boštjan J., Luzar, Boštjan
Lenguaje:	Inglés
Publicado:	PLOS 2017
Materias:	HPV204 HPV1 HPV63 Genus Mu-PV Molecular characterization
Acceso en línea:	http://hdl.handle.net/2133/9406 http://hdl.handle.net/2133/9406
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-9406
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	HPV204 HPV1 HPV63 Genus Mu-PV Molecular characterization
spellingShingle	HPV204 HPV1 HPV63 Genus Mu-PV Molecular characterization Chouhy, Diego Bolatti, Elisa María Seme, Katja Poljak, Mario Giri, Adriana Angélica Šterbenc, Anja Hošnjak, Lea Oštrbenk, Anja Kocjan, Boštjan J. Luzar, Boštjan Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.
topic_facet	HPV204 HPV1 HPV63 Genus Mu-PV Molecular characterization
description	HPV204 is the only newly identified Mupapillomavirus (Mu-PV) type in more than a decade. To comprehensively characterize HPV204, we performed a detailed molecular analysis of the viral genome and evaluated its clinical relevance in comparison to the other Mu-PVs, HPV1 and HPV63. The 7,227-bp long genome of HPV204 exhibits typical genomic organization of Mu-PVs with eight open reading frames (ORFs) (E6, E7, E1, E2, E8, E4, L2, and L1). We developed three type-specific quantitative real-time PCRs and used them to test a representative collection (n = 1,006) of various HPV-associated benign and malignant neoplasms, as well as samples of clinically normal cutaneous, mucosal, and mucocutaneous origins. HPV204, HPV1, and HPV63 were detected in 1.1%, 2.7%, and 1.9% of samples tested, respectively, and were present in skin and mucosa, suggesting dual tissue tropism of all Mu-PVs. To evaluate the etiological role of Mu-PVs in the development of HPV-associated neoplasms, Mu-PV viral loads per single cell were estimated. HPV1 and HPV63 were present in high viral copy numbers in 3/43 and 1/43 cutaneous warts, respectively, and were identified as the most likely causative agents of these warts. HPV204 viral load was extremely low in a single HPV204-positive cutaneous wart (7.4 × 10−7 viral copies/cell). Hence, etiological association between HPV204 and the development of cutaneous warts could not be established. To the best of our knowledge, this is the first study to evaluate the genetic variability of Mu-PVs by sequencing complete LCR genomic regions of HPV204, HPV1, and HPV63. We detected several nucleotide substitutions and deletions within the LCR genomic regions of Mu-PVs and identified two genetic variants of HPV204 and HPV63 and five genetic variants of HPV1.
author	Chouhy, Diego Bolatti, Elisa María Seme, Katja Poljak, Mario Giri, Adriana Angélica Šterbenc, Anja Hošnjak, Lea Oštrbenk, Anja Kocjan, Boštjan J. Luzar, Boštjan
author_facet	Chouhy, Diego Bolatti, Elisa María Seme, Katja Poljak, Mario Giri, Adriana Angélica Šterbenc, Anja Hošnjak, Lea Oštrbenk, Anja Kocjan, Boštjan J. Luzar, Boštjan
author_sort	Chouhy, Diego
title	Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.
title_short	Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.
title_full	Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.
title_fullStr	Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.
title_full_unstemmed	Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.
title_sort	molecular characterization, tissue tropism, and genetic variability of the novel mupapillomavirus type hpv204 and phylogenetically related types hpv1 and hpv63.
publisher	PLOS
publishDate	2017
url	http://hdl.handle.net/2133/9406 http://hdl.handle.net/2133/9406
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bdutipo_str	Repositorios
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Molecular characterization, tissue tropism, and genetic variability of the novel Mupapillomavirus type HPV204 and phylogenetically related types HPV1 and HPV63.

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