Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons

Tuberculosis (TB) remains the most frequent cause of illness and death from an infectious agent, and its interaction with HIV has devastating effects. We determined plasma levels of dehydroepiandrosterone (DHEA), its circulating form DHEA-suphate (DHEA-s) and cortisol in different stages of M. tuber...

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Autores principales: Quiroga, Maria Florencia, Angerami, Matias Tomas, Santucci, Natalia, Ameri, Diego, Francos, Jose Luis, Wallach, Jorge, Sued, Omar, Cahn, Pedro, Salomón, Horacio, Bottasso, Oscar
Formato: artículo article publishedVersion
Lenguaje:Inglés
Publicado: PLOS (Public Library of Science) 2014
Materias:
Tuberculosis
Blood plasma
HIV
Hydrocortisone
Immune response
Mycobacterium tuberculosis
Regulatory T cells
T cells
Acceso en línea:http://hdl.handle.net/2133/3080
http://hdl.handle.net/2133/3080
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-3080
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv en
topic Tuberculosis
Blood plasma
HIV
Hydrocortisone
Immune response
Mycobacterium tuberculosis
Regulatory T cells
T cells
spellingShingle Tuberculosis
Blood plasma
HIV
Hydrocortisone
Immune response
Mycobacterium tuberculosis
Regulatory T cells
T cells
Quiroga, Maria Florencia
Angerami, Matias Tomas
Santucci, Natalia
Ameri, Diego
Francos, Jose Luis
Wallach, Jorge
Sued, Omar
Cahn, Pedro
Salomón, Horacio
Bottasso, Oscar
Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons
topic_facet Tuberculosis
Blood plasma
HIV
Hydrocortisone
Immune response
Mycobacterium tuberculosis
Regulatory T cells
T cells
description Tuberculosis (TB) remains the most frequent cause of illness and death from an infectious agent, and its interaction with HIV has devastating effects. We determined plasma levels of dehydroepiandrosterone (DHEA), its circulating form DHEA-suphate (DHEA-s) and cortisol in different stages of M. tuberculosis infection, and explored their role on the Th1 and Treg populations during different scenarios of HIV-TB coinfection, including the immune reconstitution inflammatory syndrome (IRIS), a condition related to antiretroviral treatment. DHEA levels were diminished in HIV-TB and HIV-TB IRIS patients compared to healthy donors (HD), HIV+ individuals and HIV+ individuals with latent TB (HIV-LTB), whereas dehydroepiandrosterone sulfate (DHEA-s) levels were markedly diminished in HIV-TB IRIS individuals. HIV-TB and IRIS patients presented a cortisol/DHEA ratio significantly higher than HIV+, HIV-LTB and HD individuals. A positive correlation was observed between DHEA-s and CD4 count among HIV-TB individuals. Conversely, cortisol plasma level inversely correlated with CD4 count within HIV-TB individuals. M. tuberculosis-specific Th1 lymphocyte count was increased after culturing PBMC from HIV-TB individuals in presence of DHEA. We observed an inverse correlation between DHEA-s plasma level and Treg frequency in co-infected individuals, and CD4+FoxP3+ Treg frequency was increased in HIV-TB and IRIS patients compared to other groups. Strikingly, we observed a prominent CD4+CD25-FoxP3+ population across HIV-TB and HIV-TB IRIS patients, which frequency correlated with DHEA plasma level. Finally, DHEA treatment negatively regulated FoxP3 expression without altering Treg frequency in co-infected patients. These data suggest an enhancing role for DHEA in the immune response against M. tuberculosis during HIV-TB coinfection and IRIS.
format artículo
article
publishedVersion
author Quiroga, Maria Florencia
Angerami, Matias Tomas
Santucci, Natalia
Ameri, Diego
Francos, Jose Luis
Wallach, Jorge
Sued, Omar
Cahn, Pedro
Salomón, Horacio
Bottasso, Oscar
author_facet Quiroga, Maria Florencia
Angerami, Matias Tomas
Santucci, Natalia
Ameri, Diego
Francos, Jose Luis
Wallach, Jorge
Sued, Omar
Cahn, Pedro
Salomón, Horacio
Bottasso, Oscar
author_sort Quiroga, Maria Florencia
title Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons
title_short Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons
title_full Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons
title_fullStr Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons
title_full_unstemmed Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons
title_sort dynamics of adrenal steroids are related to variations in th1 and treg populations during mycobacterium tuberculosis infection in hiv positive persons
publisher PLOS (Public Library of Science)
publishDate 2014
url http://hdl.handle.net/2133/3080
http://hdl.handle.net/2133/3080
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