Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii

According to the Centers for Disease Control and Prevention, Acinetobacter baumannii is listed among the most threatening pathogens. A. baumannii is mainly a nosocomial pathogen with a distinctive ability to survive in multiple environments. These characteristics together with this bacterium’s ab...

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Autores principales: Escalante, Jenny, Nishimura, Brent, Tuttobene, Marisel Romina, Subils, Tomás, Pimentel, Camila, Georgeos, Nardin, Sieira, Rodrigo, Bonomo, Robert A., Tolmasky, Marcelo E., Ramírez, María Soledad
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Nature Research 2023
Materias:
Acinetobacter baumannii
Anti-bacterial agents
Bacterial proteins
Biofilms
Gene expression regulation
Bacterial
Acceso en línea:http://hdl.handle.net/2133/25135
http://hdl.handle.net/2133/25135
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-25135
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Acinetobacter baumannii
Anti-bacterial agents
Bacterial proteins
Biofilms
Gene expression regulation
Bacterial
spellingShingle Acinetobacter baumannii
Anti-bacterial agents
Bacterial proteins
Biofilms
Gene expression regulation
Bacterial
Escalante, Jenny
Nishimura, Brent
Tuttobene, Marisel Romina
Subils, Tomás
Pimentel, Camila
Georgeos, Nardin
Sieira, Rodrigo
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramírez, María Soledad
Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii
topic_facet Acinetobacter baumannii
Anti-bacterial agents
Bacterial proteins
Biofilms
Gene expression regulation
Bacterial
description According to the Centers for Disease Control and Prevention, Acinetobacter baumannii is listed among the most threatening pathogens. A. baumannii is mainly a nosocomial pathogen with a distinctive ability to survive in multiple environments. These characteristics together with this bacterium’s ability to acquire antibiotic resistance determinants make it a notorious pathogen. The presence of human serum albumin (HSA) is associated with modifcation of expression levels in numerous genes. The presence of HSA in the culture medium is also correlated with a reduction in levels of the global suppressor histone-like nucleoid structure protein, H-NS. Comparative transcriptome analysis of the wild type and isogenic Δhns strains cultured in lysogeny broth (LB) in the presence or absence of HSA revealed that the expression of a subset of eleven genes are modifed in the Δhns cultured in LB and the wild-type strain in the presence of HSA, pointing out these genes as candidates to be regulated by the presence of HSA through H-NS. Six and fve of these genes were up- or down-regulated, respectively. Three of these genes have functions in quorum sensing (acdA, kar and fadD), one in quorum quenching (aidA), two in stress response (katE, ywrO), three in metabolism (phaC, yedL1, and yedL2), one in bioflm formation (csuAB), and one in β-oxidation of fatty acids (fadA). The regulation of these genes was assessed by: (i) transcriptional analysis and qPCR at the transcriptional level; and (ii) by determining the phenotypic characteristics of each function. The results of these studies support the hypothesis that HSA-mediated reduction of H-NS levels may be one very important regulatory circuit utilized by A. baumannii to adapt to selected environments, such as those where HSAcontaining human fuids are abundant.
format article
artículo
publishedVersion
author Escalante, Jenny
Nishimura, Brent
Tuttobene, Marisel Romina
Subils, Tomás
Pimentel, Camila
Georgeos, Nardin
Sieira, Rodrigo
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramírez, María Soledad
author_facet Escalante, Jenny
Nishimura, Brent
Tuttobene, Marisel Romina
Subils, Tomás
Pimentel, Camila
Georgeos, Nardin
Sieira, Rodrigo
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramírez, María Soledad
author_sort Escalante, Jenny
title Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii
title_short Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii
title_full Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii
title_fullStr Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii
title_full_unstemmed Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii
title_sort human serum albumin (hsa) regulates the expression of histone-like nucleoid structure protein (h-ns) in acinetobacter baumannii
publisher Nature Research
publishDate 2023
url http://hdl.handle.net/2133/25135
http://hdl.handle.net/2133/25135
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