A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix

Huanglongbing (HLB), the current major threat for Citrus species, is caused by intracellular alphaproteobacteria of the genus Candidatus Liberibacter (CaL), with CaL asiaticus (CLas) being the most prevalent species. This bacterium inhabits phloem cells and is transmitted by the psyllid Diaphorin...

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Autores principales: García, Lucila, Molina, María Celeste, Padgett Pagliai, Kaylie Allyson, Torres, Pablo S., Bruna, Roberto Emanuel, García Véscovi, Eleonora, González, Claudio F., Gadea, José, Marano, María Rosa
Lenguaje:Inglés
Publicado: 2022
Materias:
Biofilm
Huanglongbing
Protease
Surrogate bacteria
Virulence factor
Acceso en línea:http://hdl.handle.net/2133/25033
http://hdl.handle.net/2133/25033
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-25033
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Biofilm
Huanglongbing
Protease
Surrogate bacteria
Virulence factor
spellingShingle Biofilm
Huanglongbing
Protease
Surrogate bacteria
Virulence factor
García, Lucila
Molina, María Celeste
Padgett Pagliai, Kaylie Allyson
Torres, Pablo S.
Bruna, Roberto Emanuel
García Véscovi, Eleonora
González, Claudio F.
Gadea, José
Marano, María Rosa
A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix
topic_facet Biofilm
Huanglongbing
Protease
Surrogate bacteria
Virulence factor
description Huanglongbing (HLB), the current major threat for Citrus species, is caused by intracellular alphaproteobacteria of the genus Candidatus Liberibacter (CaL), with CaL asiaticus (CLas) being the most prevalent species. This bacterium inhabits phloem cells and is transmitted by the psyllid Diaphorina citri. A gene encoding a putative serralysin-like metalloprotease (CLIBASIA_01345) was identified in the CLas genome. The expression levels of this gene were found to be higher in citrus leaves than in psyllids, suggesting a function for this protease in adaptation to the plant environment. Here, we study the putative role of CLas-serralysin (Las1345) as virulence factor. We first assayed whether Las1345 could be secreted by two different surrogate bacteria, Rhizobium leguminosarum bv. viciae A34 (A34) and Serratia marcescens. The protein was detected only in the cellular fraction of A34 and S. marcescens expressing Las1345, and increased protease activity of those bacteria by 2.55 and 4.25- fold, respectively. In contrast, Las1345 expressed in Nicotiana benthamiana leaves did not show protease activity nor alterations in the cell membrane, suggesting that Las1345 do not function as a protease in the plant cell. Las1345 expression negatively regulated cell motility, exopolysaccharide production, and biofilm formation in Xanthomonas campestris pv. campestris (Xcc). This bacterial phenotype was correlated with reduced growth and survival on leaf surfaces as well as reduced disease symptoms in N. benthamiana and Arabidopsis. These results support a model where Las1345 could modify extracellular components to adapt bacterial shape and appendages to the phloem environment, thus contributing to virulence.
author García, Lucila
Molina, María Celeste
Padgett Pagliai, Kaylie Allyson
Torres, Pablo S.
Bruna, Roberto Emanuel
García Véscovi, Eleonora
González, Claudio F.
Gadea, José
Marano, María Rosa
author_facet García, Lucila
Molina, María Celeste
Padgett Pagliai, Kaylie Allyson
Torres, Pablo S.
Bruna, Roberto Emanuel
García Véscovi, Eleonora
González, Claudio F.
Gadea, José
Marano, María Rosa
author_sort García, Lucila
title A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix
title_short A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix
title_full A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix
title_fullStr A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix
title_full_unstemmed A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix
title_sort serralysin-like protein of candidatus liberibacter asiaticus modulates components of the bacterial extracellular matrix
publishDate 2022
url http://hdl.handle.net/2133/25033
http://hdl.handle.net/2133/25033
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