Evolution of Copper Homeostasis and Virulence in Salmonella

Salmonella enterica sv. Typhimurium modulates the expression of factors essential for virulence, contributing to its survival against the surge of copper (Cu) in the Salmonella-containing vacuole. This bactericidal host innate immune component primarily targets the bacterial envelope, where most cup...

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Autores principales: Méndez, Andrea A. E., Mendoza, Julián I., Echarren, María Laura, Terán, Ignacio, Checa, Susana Karina, Soncini, Fernando C.
Lenguaje:Inglés
Publicado: Frontiers Media 2022
Materias:
Copper
Bacterial envelope
CueP
CusCFBA
Host-pathogen interaction
Acceso en línea:http://hdl.handle.net/2133/23694
http://hdl.handle.net/2133/23694
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-23694
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Copper
Bacterial envelope
CueP
CusCFBA
Host-pathogen interaction
spellingShingle Copper
Bacterial envelope
CueP
CusCFBA
Host-pathogen interaction
Méndez, Andrea A. E.
Mendoza, Julián I.
Echarren, María Laura
Terán, Ignacio
Checa, Susana Karina
Soncini, Fernando C.
Evolution of Copper Homeostasis and Virulence in Salmonella
topic_facet Copper
Bacterial envelope
CueP
CusCFBA
Host-pathogen interaction
description Salmonella enterica sv. Typhimurium modulates the expression of factors essential for virulence, contributing to its survival against the surge of copper (Cu) in the Salmonella-containing vacuole. This bactericidal host innate immune component primarily targets the bacterial envelope, where most cuproproteins are localized. While in most enteric species periplasmic Cu homeostasis is maintained by the CusR/CusS-controlled CusCFBA efflux system encoded in the cus locus, we noticed that these genes were lost from the Salmonella-core genome. At the same time, Salmonella acquired cueP, coding for a periplasmic Cu chaperone. As cus, cueP was shown to be essential for bacterial survival in a copper-rich environment under anaerobiosis, suggesting that it can functionally substitute the CusCFBA system. In the present study, the whole Escherichia coli cus locus was reintroduced to the chromosome of the Salmonella wild-type or th 1cueP strain. While the integrated cus locus did not affect Cu resistance under aerobic conditions, it increases Cu tolerance under anaerobiosis, irrespective of the presence or absence of cueP. In contrast to the Cus system, CueP expression is higher at high copper concentrations and persisted over time, suggesting separate functions. Finally, we observed that, regardless of the presence or absence of cus, a mutant deleted of cueP shows a deficiency in replication inside macrophages compared to the wild- type strain. Our results demonstrate that CueP and CusCFBA exert redundant functions for metal resistance, but not for intracellular survival, and therefore for the virulence of this pathogen.
author Méndez, Andrea A. E.
Mendoza, Julián I.
Echarren, María Laura
Terán, Ignacio
Checa, Susana Karina
Soncini, Fernando C.
author_facet Méndez, Andrea A. E.
Mendoza, Julián I.
Echarren, María Laura
Terán, Ignacio
Checa, Susana Karina
Soncini, Fernando C.
author_sort Méndez, Andrea A. E.
title Evolution of Copper Homeostasis and Virulence in Salmonella
title_short Evolution of Copper Homeostasis and Virulence in Salmonella
title_full Evolution of Copper Homeostasis and Virulence in Salmonella
title_fullStr Evolution of Copper Homeostasis and Virulence in Salmonella
title_full_unstemmed Evolution of Copper Homeostasis and Virulence in Salmonella
title_sort evolution of copper homeostasis and virulence in salmonella
publisher Frontiers Media
publishDate 2022
url http://hdl.handle.net/2133/23694
http://hdl.handle.net/2133/23694
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