Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes

The Pseudomonas putida group (P. putida G) is composed of at least 21 species associated with a wide range of environments, including the clinical setting. Here, we characterized 13 carbapenem-resistant P. putida G clinical isolates bearing class 1 integrons/transposons (class 1 In/Tn) carrying blaV...

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Autores principales: Brovedan, Marco Alcides, Marchiaro, Patricia M., Díaz, María S., Faccone, Diego, Corso, Alejandra, Pasteran, Fernando, Viale, Alejandro M., Limansky, Adriana S.
Lenguaje:Inglés
Publicado: Elsevier B.V. 2022
Materias:
Pseudomonas putida group
Carbapenem resistance plasmids
Tn402-like class 1 integrons
VIM-2 metallo-β-lactamase reservoirs
Mobile genetic elements
Tn21
Acceso en línea:http://hdl.handle.net/2133/23644
http://hdl.handle.net/2133/23644
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-23644
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Pseudomonas putida group
Carbapenem resistance plasmids
Tn402-like class 1 integrons
VIM-2 metallo-β-lactamase reservoirs
Mobile genetic elements
Tn21
spellingShingle Pseudomonas putida group
Carbapenem resistance plasmids
Tn402-like class 1 integrons
VIM-2 metallo-β-lactamase reservoirs
Mobile genetic elements
Tn21
Brovedan, Marco Alcides
Marchiaro, Patricia M.
Díaz, María S.
Faccone, Diego
Corso, Alejandra
Pasteran, Fernando
Viale, Alejandro M.
Limansky, Adriana S.
Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
topic_facet Pseudomonas putida group
Carbapenem resistance plasmids
Tn402-like class 1 integrons
VIM-2 metallo-β-lactamase reservoirs
Mobile genetic elements
Tn21
description The Pseudomonas putida group (P. putida G) is composed of at least 21 species associated with a wide range of environments, including the clinical setting. Here, we characterized 13 carbapenem-resistant P. putida G clinical isolates bearing class 1 integrons/transposons (class 1 In/Tn) carrying blaVIM-2 metallo-β-lactamase gene cassettes obtained from hospitals of Argentina. Multilocus sequencing (MLSA) and phylogenetic analyses based on 16S rDNA, gyrB and rpoD sequences distinguished 7 species among them. blaVIM-2 was found in three different cassette arrays: In41 (blaVIM-2-aacA4), In899 (only blaVIM-2), and In528 (dfrB1-aacA4-blaVIM-2). In41 and In899 were associated with complete tniABQC transposition modules and IRi/IRt boundaries characteristic of the Tn5053/Tn402 transposons, which were designated Tn6335 and Tn6336, respectively. The class 1 In/Tn element carrying In528, however, exhibited a defective tni module bearing only the tniC (transposase) gene, associated with a complete IS6100 bounded with two oppositely-oriented IRt end regions. In some P. putida G isolates including P. asiatica, P. juntendi, P. putida G/II, and P. putida G/V, Tn6335/Tn6336 were carried by pLD209-type conjugative plasmids capable of self-mobilization to P. aeruginosa or Escherichia coli. In other isolates of P. asiatica, P. putida G/II, and P. monteiliieilii, however, these blaVIM-2-containing class 1 In/Tn elements were found inserted into the res regions preceding the tnpR (resolvase) gene of particular Tn21 subgroup members of Tn3 transposons. The overall results reinforce the notion of P. putida G members as blaVIM-2 reservoirs, and shed light on the mechanisms of dissemination of carbapenem resistance genes to other pathogenic bacteria in the clinical setting.
author Brovedan, Marco Alcides
Marchiaro, Patricia M.
Díaz, María S.
Faccone, Diego
Corso, Alejandra
Pasteran, Fernando
Viale, Alejandro M.
Limansky, Adriana S.
author_facet Brovedan, Marco Alcides
Marchiaro, Patricia M.
Díaz, María S.
Faccone, Diego
Corso, Alejandra
Pasteran, Fernando
Viale, Alejandro M.
Limansky, Adriana S.
author_sort Brovedan, Marco Alcides
title Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
title_short Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
title_full Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
title_fullStr Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
title_full_unstemmed Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
title_sort pseudomonas putida group species as reservoirs of mobilizable tn402-like class 1 integrons carrying blavim-2 metallo-β-lactamase genes
publisher Elsevier B.V.
publishDate 2022
url http://hdl.handle.net/2133/23644
http://hdl.handle.net/2133/23644
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