Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus

Hemicellulose is a major component of plant cell walls and xylan is the most predominant polysaccharide. Xylan degrading enzymes integrate the xylanolytic system. Xylanolytic enzymes were produced by fermentation by Aspergillus niger and Thermomyces lanuginosus grown on grass considered unsuitable f...

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Autores principales: Pellieri, Carlos Martín, Taddia, Antonela, Loureiro, Dana Belén, Bortolato, Santiago Andrés, Tubio, Gisela
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Elsevier 2022
Materias:
Bioprocesses
Lignocellulosic Biomass
Fungal Fermentation
Enzyme Statistical Design Production
Sustainability
Circular Economy
Acceso en línea:http://hdl.handle.net/2133/23373
http://hdl.handle.net/2133/23373
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-23373
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Bioprocesses
Lignocellulosic Biomass
Fungal Fermentation
Enzyme Statistical Design Production
Sustainability
Circular Economy
spellingShingle Bioprocesses
Lignocellulosic Biomass
Fungal Fermentation
Enzyme Statistical Design Production
Sustainability
Circular Economy
Pellieri, Carlos Martín
Taddia, Antonela
Loureiro, Dana Belén
Bortolato, Santiago Andrés
Tubio, Gisela
Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus
topic_facet Bioprocesses
Lignocellulosic Biomass
Fungal Fermentation
Enzyme Statistical Design Production
Sustainability
Circular Economy
description Hemicellulose is a major component of plant cell walls and xylan is the most predominant polysaccharide. Xylan degrading enzymes integrate the xylanolytic system. Xylanolytic enzymes were produced by fermentation by Aspergillus niger and Thermomyces lanuginosus grown on grass considered unsuitable for farming called Spartina argentinensis. Significant parameters: type of fermentation used (SmF or SSF), type of leaves of S. argentinensis (green or senescent) and conidia final concentration for xylanase production were screened and optimized. The main results showed that the highest levels of xylanolytic enzyme production were obtained by 1 × 105 conidia/mL of A. niger in SmF at 96 h, 30ºC, with a mixture of 20.20% of senescent and 79.80% of green leaves. The xylanase specific activity obtained was 62 U/mg, higher than the activity obtained (23 U/mg) in previous work (Taddia et al., 2019), and the concentration of xylanolytic production over that of glucanase activity was maximized five times. The optimized enzymatic extract obtained was characterized by LC-MS and HPLC of carbohydrates. Six enzymes were identified as constituents of the xylanolytic complex and seven carbohydrates. Moreover, the xylanolytic enzyme extract was stable for 30 days at 20 °C. Thus, S. argentinensis can be used within the framework of a circular economy, rendering a synergistic combination of the xylanolytic enzymes with industrial applications.
format article
artículo
publishedVersion
author Pellieri, Carlos Martín
Taddia, Antonela
Loureiro, Dana Belén
Bortolato, Santiago Andrés
Tubio, Gisela
author_facet Pellieri, Carlos Martín
Taddia, Antonela
Loureiro, Dana Belén
Bortolato, Santiago Andrés
Tubio, Gisela
author_sort Pellieri, Carlos Martín
title Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus
title_short Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus
title_full Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus
title_fullStr Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus
title_full_unstemmed Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus
title_sort spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus
publisher Elsevier
publishDate 2022
url http://hdl.handle.net/2133/23373
http://hdl.handle.net/2133/23373
work_keys_str_mv AT pelliericarlosmartin spartinaargentinensisvalorizationandprocessoptimizationforenhancedproductionofhydrolyticenzymesbyfilamentousfungus
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bdutipo_str Repositorios
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