Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses

Bats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, th...

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Autores principales: Bolatti, Elisa María, Viarengo, Gastón, Zorec, Tomaž M, Cerri, Agustina, Montani, María E., Hošnjak, Lea, Casal, Pablo E., Bortolotto, Eugenia, Di Domenica, Violeta, Chouhy, Diego, Allasia, María Belén, Barquez, Rubén M., Poljak, Mario, Giri, Adriana Angélica
Lenguaje:Inglés
Publicado: MDPI 2022
Materias:
Chiroptera
Metagenomic
Virome
Virus identification
Cressdnaviricota
Cossaviricota
Anelloviridae
Acceso en línea:http://hdl.handle.net/2133/23357
http://hdl.handle.net/2133/23357
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-23357
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Chiroptera
Metagenomic
Virome
Virus identification
Cressdnaviricota
Cossaviricota
Anelloviridae
spellingShingle Chiroptera
Metagenomic
Virome
Virus identification
Cressdnaviricota
Cossaviricota
Anelloviridae
Bolatti, Elisa María
Viarengo, Gastón
Zorec, Tomaž M
Cerri, Agustina
Montani, María E.
Hošnjak, Lea
Casal, Pablo E.
Bortolotto, Eugenia
Di Domenica, Violeta
Chouhy, Diego
Allasia, María Belén
Barquez, Rubén M.
Poljak, Mario
Giri, Adriana Angélica
Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses
topic_facet Chiroptera
Metagenomic
Virome
Virus identification
Cressdnaviricota
Cossaviricota
Anelloviridae
description Bats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, the diversity of DNA viruses of five arthropodophagous bat species from Argentina was investigated using metagenomics. Fecal samples of 29 individuals from five species (Tadarida brasiliensis, Molossus molossus, Eumops bonariensis, Eumops patagonicus, and Eptesicus diminutus) living at two different geographical locations, were investigated. Enriched viral DNA was sequenced using Illumina MiSeq, and the reads were trimmed and filtered using several bioinformatic approaches. The resulting nucleotide sequences were subjected to viral taxonomic classification. In total, 4,520,370 read pairs were sequestered by sequencing, and 21.1% of them mapped to viral taxa. Circoviridae and Genomoviridae were the most prevalent among vertebrate viral families in all bat species included in this study. Samples from the T. brasiliensis colony exhibited lower viral diversity than samples from other species of New World bats. We characterized 35 complete genome sequences of novel viruses. These findings provide new insights into the global diversity of bat viruses in poorly studied species, contributing to prevention of emerging zoonotic diseases and to conservation policies for endangered species.
author Bolatti, Elisa María
Viarengo, Gastón
Zorec, Tomaž M
Cerri, Agustina
Montani, María E.
Hošnjak, Lea
Casal, Pablo E.
Bortolotto, Eugenia
Di Domenica, Violeta
Chouhy, Diego
Allasia, María Belén
Barquez, Rubén M.
Poljak, Mario
Giri, Adriana Angélica
author_facet Bolatti, Elisa María
Viarengo, Gastón
Zorec, Tomaž M
Cerri, Agustina
Montani, María E.
Hošnjak, Lea
Casal, Pablo E.
Bortolotto, Eugenia
Di Domenica, Violeta
Chouhy, Diego
Allasia, María Belén
Barquez, Rubén M.
Poljak, Mario
Giri, Adriana Angélica
author_sort Bolatti, Elisa María
title Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_short Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_full Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_fullStr Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_full_unstemmed Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses
title_sort viral metagenomic data analyses of five new world bat species from argentina: identification of 35 novel dna viruses
publisher MDPI
publishDate 2022
url http://hdl.handle.net/2133/23357
http://hdl.handle.net/2133/23357
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bdutipo_str Repositorios
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