Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain

Hyaluronic acid (HA) is a high value glycosaminoglycan mostly used in health and cosmetic applications. Commercial HA is produced from animal tissues or in toxigenic bacteria of the genus Streptococcus grown in complex media, which are expensive and raise environmental concerns due to the disposal o...

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Autores principales: Cerminati, Sebastián, Leroux, Mélanie, Anselmi, Pablo Ariel, Peirú, Salvador, Alonso, Juan C., Priem, Bernard, Menzella, Hugo G.
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Springer 2022
Materias:
Hyaluronic acid
Green chemistry
Bacillus subtilis
Fermentation
Acceso en línea:http://hdl.handle.net/2133/23283
http://hdl.handle.net/2133/23283
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-23283
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Hyaluronic acid
Green chemistry
Bacillus subtilis
Fermentation
spellingShingle Hyaluronic acid
Green chemistry
Bacillus subtilis
Fermentation
Cerminati, Sebastián
Leroux, Mélanie
Anselmi, Pablo Ariel
Peirú, Salvador
Alonso, Juan C.
Priem, Bernard
Menzella, Hugo G.
Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain
topic_facet Hyaluronic acid
Green chemistry
Bacillus subtilis
Fermentation
description Hyaluronic acid (HA) is a high value glycosaminoglycan mostly used in health and cosmetic applications. Commercial HA is produced from animal tissues or in toxigenic bacteria of the genus Streptococcus grown in complex media, which are expensive and raise environmental concerns due to the disposal of large amounts of broth with high organic loads. Other microorganisms were proposed as hosts for the heterologous production of HA, but the methods are still costly. The extraordinary capacity of this biopolymer to bind and retain water attracts interest for large-scale applications where biodegradable materials are needed, but its high cost and safety concerns are barriers for its adoption. Bacillus subtilis 3NA strain is prototrophic, amenable for genetic manipulation, GRAS, and can rapidly reach high cell densities in salt-based media. These phenotypic traits were exploited to create a platform for biomolecule production using HA as a proof of concept. First, the 3NA strain was engineered to produce HA; second, a chemically defined medium was formulated using commodity-priced inorganic salts combined at the stoichiometric ratios needed to build the necessary quantities of biomass and HA; and third, a scalable fermentation process, where HA can be produced at the maximum volumetric productivity (VP), was designed. A comparative economic analysis against other methods indicates that the new process may increase the operating profit of a manufacturing plant by more than 100%. The host, the culture medium, and the rationale employed to develop the fermentation process described here, introduce an IP free platform that could be adaptable for production of other biomolecules.
format article
artículo
publishedVersion
author Cerminati, Sebastián
Leroux, Mélanie
Anselmi, Pablo Ariel
Peirú, Salvador
Alonso, Juan C.
Priem, Bernard
Menzella, Hugo G.
author_facet Cerminati, Sebastián
Leroux, Mélanie
Anselmi, Pablo Ariel
Peirú, Salvador
Alonso, Juan C.
Priem, Bernard
Menzella, Hugo G.
author_sort Cerminati, Sebastián
title Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain
title_short Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain
title_full Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain
title_fullStr Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain
title_full_unstemmed Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain
title_sort low cost and sustainable hyaluronic acid production in a manufacturing platform based on bacillus subtilis 3na strain
publisher Springer
publishDate 2022
url http://hdl.handle.net/2133/23283
http://hdl.handle.net/2133/23283
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bdutipo_str Repositorios
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