Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development

The neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. A number of gene regulatory networks coordinating neural crest cell specification and differentiation have be...

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Autores principales:	Steeman, Tomás José, Rubiolo, Juan Andrés, Sánchez, Laura E., Calcaterra, Nora B., Weiner, Andrea María Julia
Formato:	article artículo
Lenguaje:	Inglés
Publicado:	MDPI 2022
Materias:	MicroRNA Neural Crest Gene Regulatory Network Embryonic Developmen
Acceso en línea:	http://hdl.handle.net/2133/23272 http://hdl.handle.net/2133/23272
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-23272
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	MicroRNA Neural Crest Gene Regulatory Network Embryonic Developmen
spellingShingle	MicroRNA Neural Crest Gene Regulatory Network Embryonic Developmen Steeman, Tomás José Rubiolo, Juan Andrés Sánchez, Laura E. Calcaterra, Nora B. Weiner, Andrea María Julia Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
topic_facet	MicroRNA Neural Crest Gene Regulatory Network Embryonic Developmen
description	The neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. A number of gene regulatory networks coordinating neural crest cell specification and differentiation have been extensively studied to date. Although several publications suggest a common role for microRNA-145 (miR-145) in molecular reprogramming for cell cycle regulation and/or cellular differ entiation, little is known about its role during in vivo cranial neural crest development. By modifying miR-145 levels in zebrafish embryos, abnormal craniofacial development and aberrant pigmentation phenotypes were detected. By whole-mount in situ hybridization, changes in expression patterns of col2a1a and Sry-related HMG box (Sox) transcription factors sox9a and sox9b were observed in overexpressed miR-145 embryos. In agreement, zebrafish sox9b expression was downregulated by miR-145 overexpression. In silico and in vivo analysis of the sox9b 3 0UTR revealed a conserved potential miR-145 binding site likely involved in its post-transcriptional regulation. Based on these findings, we speculate that miR-145 participates in the gene regulatory network governing zebrafish chondrocyte differentiation by controlling sox9b expression.
format	article artículo
author	Steeman, Tomás José Rubiolo, Juan Andrés Sánchez, Laura E. Calcaterra, Nora B. Weiner, Andrea María Julia
author_facet	Steeman, Tomás José Rubiolo, Juan Andrés Sánchez, Laura E. Calcaterra, Nora B. Weiner, Andrea María Julia
author_sort	Steeman, Tomás José
title	Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_short	Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_full	Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_fullStr	Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_full_unstemmed	Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
title_sort	conservation of zebrafish microrna-145 and its role during neural crest cell development
publisher	MDPI
publishDate	2022
url	http://hdl.handle.net/2133/23272 http://hdl.handle.net/2133/23272
work_keys_str_mv	AT steemantomasjose conservationofzebrafishmicrorna145anditsroleduringneuralcrestcelldevelopment AT rubiolojuanandres conservationofzebrafishmicrorna145anditsroleduringneuralcrestcelldevelopment AT sanchezlaurae conservationofzebrafishmicrorna145anditsroleduringneuralcrestcelldevelopment AT calcaterranorab conservationofzebrafishmicrorna145anditsroleduringneuralcrestcelldevelopment AT weinerandreamariajulia conservationofzebrafishmicrorna145anditsroleduringneuralcrestcelldevelopment
bdutipo_str	Repositorios
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Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development

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