Methods: A new protocol for in vitro red blood cell glycation

During diabetes, the characteristic hyperglycemia can induce red blood cell glycation. Several researchers have proposed different protocols to perform an in vitro model to study this phenomenon. In this article, some of the most important in vitro glycation protocols available in the bibliography w...

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Autores principales: Batista da Silva, Marcus V., Alet, Analía I., Castellini, Horacio V., Riquelme, Bibiana Doris
Formato: article artículo acceptedVersion
Lenguaje:Inglés
Publicado: Elsevier 2021
Materias:
High blood sugar
Non-enzymatic glycation
In vitro protocol
Glucose incubation
Erythrocyte glycation
Acceso en línea:http://hdl.handle.net/2133/22972
http://hdl.handle.net/2133/22972
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-22972
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic High blood sugar
Non-enzymatic glycation
In vitro protocol
Glucose incubation
Erythrocyte glycation
spellingShingle High blood sugar
Non-enzymatic glycation
In vitro protocol
Glucose incubation
Erythrocyte glycation
Batista da Silva, Marcus V.
Alet, Analía I.
Castellini, Horacio V.
Riquelme, Bibiana Doris
Methods: A new protocol for in vitro red blood cell glycation
topic_facet High blood sugar
Non-enzymatic glycation
In vitro protocol
Glucose incubation
Erythrocyte glycation
description During diabetes, the characteristic hyperglycemia can induce red blood cell glycation. Several researchers have proposed different protocols to perform an in vitro model to study this phenomenon. In this article, some of the most important in vitro glycation protocols available in the bibliography were compared to each other. The incubation parameters as the suspension medium, glucose concentration, red blood cell concentration, time, and temperature were analyzed. Also, several assays were carried out in our laboratory, and glycated hemoglobin, erythrocyte aggregation and viscoelasticity were determined for the protocol validation. Based on the bibliographic analysis and our experimental results, an optimal protocol for in vitro glycation of red blood cells is presented.
format article
artículo
acceptedVersion
author Batista da Silva, Marcus V.
Alet, Analía I.
Castellini, Horacio V.
Riquelme, Bibiana Doris
author_facet Batista da Silva, Marcus V.
Alet, Analía I.
Castellini, Horacio V.
Riquelme, Bibiana Doris
author_sort Batista da Silva, Marcus V.
title Methods: A new protocol for in vitro red blood cell glycation
title_short Methods: A new protocol for in vitro red blood cell glycation
title_full Methods: A new protocol for in vitro red blood cell glycation
title_fullStr Methods: A new protocol for in vitro red blood cell glycation
title_full_unstemmed Methods: A new protocol for in vitro red blood cell glycation
title_sort methods: a new protocol for in vitro red blood cell glycation
publisher Elsevier
publishDate 2021
url http://hdl.handle.net/2133/22972
http://hdl.handle.net/2133/22972
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AT castellinihoraciov methodsanewprotocolforinvitroredbloodcellglycation
AT riquelmebibianadoris methodsanewprotocolforinvitroredbloodcellglycation
bdutipo_str Repositorios
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