Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome seque...
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| Autores principales: | , , , , , , , , |
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| Formato: | publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Taylor & Francis
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/2133/20527 http://hdl.handle.net/2133/20527 |
| Aporte de: |
| Sumario: | Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are
urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on
potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections
secondary to ventilator-associated pneumonia from demographics and clinically homogeneous
patients, who received optimal treatment but with different clinical outcomes. Thus, the carO
gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from
non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the viru lence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and
a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells
and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer
membrane protein as a potential target for new therapies against CRAB infections. |
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