Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii

Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome seque...

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Autores principales: Labrador-Herrera, Gema, Pérez-Pulido, Antonio J., Álvarez-Marína, Rocío, Casimiro-Soriguer, Carlos S., Cebrero-Cangueiro, Tania, Morán-Barrio, Jorgelina, Pachón, Jerónimo, Viale, Alejandro M., Pachón-Ibáñez, María Eugenia
Formato: publishedVersion
Lenguaje:Inglés
Publicado: Taylor & Francis 2021
Materias:
Acinetobacter baumannii
Bloodstream Infections
Carbapenems Resistance
Whole-genome Sequencing
Virulence Factors
CarO
Acceso en línea:http://hdl.handle.net/2133/20527
http://hdl.handle.net/2133/20527
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20527
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Acinetobacter baumannii
Bloodstream Infections
Carbapenems Resistance
Whole-genome Sequencing
Virulence Factors
CarO
spellingShingle Acinetobacter baumannii
Bloodstream Infections
Carbapenems Resistance
Whole-genome Sequencing
Virulence Factors
CarO
Labrador-Herrera, Gema
Pérez-Pulido, Antonio J.
Álvarez-Marína, Rocío
Casimiro-Soriguer, Carlos S.
Cebrero-Cangueiro, Tania
Morán-Barrio, Jorgelina
Pachón, Jerónimo
Viale, Alejandro M.
Pachón-Ibáñez, María Eugenia
Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
topic_facet Acinetobacter baumannii
Bloodstream Infections
Carbapenems Resistance
Whole-genome Sequencing
Virulence Factors
CarO
description Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the viru lence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.
format publishedVersion
author Labrador-Herrera, Gema
Pérez-Pulido, Antonio J.
Álvarez-Marína, Rocío
Casimiro-Soriguer, Carlos S.
Cebrero-Cangueiro, Tania
Morán-Barrio, Jorgelina
Pachón, Jerónimo
Viale, Alejandro M.
Pachón-Ibáñez, María Eugenia
author_facet Labrador-Herrera, Gema
Pérez-Pulido, Antonio J.
Álvarez-Marína, Rocío
Casimiro-Soriguer, Carlos S.
Cebrero-Cangueiro, Tania
Morán-Barrio, Jorgelina
Pachón, Jerónimo
Viale, Alejandro M.
Pachón-Ibáñez, María Eugenia
author_sort Labrador-Herrera, Gema
title Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_short Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_full Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_fullStr Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_full_unstemmed Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_sort virulence role of the outer membrane protein caro in carbapenem-resistant acinetobacter baumannii
publisher Taylor & Francis
publishDate 2021
url http://hdl.handle.net/2133/20527
http://hdl.handle.net/2133/20527
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