Acinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms

Acinetobacter baumannii represents nowadays an important nosocomial pathogen of poorly defined reservoirs outside the clinical setting. Here, we con ducted whole-genome sequencing analysis of the Acinetobacter sp. NCIMB8209 col lection strain, isolated in 1943 from the aerobic degradation (retting)...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Repizo, Guillermo Daniel, Espariz, Martín, Seravalle, Joana L., Díaz Miloslavich, Juan Ignacio, Steimbrüch, Bruno A., Shuman, Howard A., Viale, Alejandro M.
Formato: publishedVersion
Lenguaje:Inglés
Publicado: American Society for Microbiology 2021
Materias:
Acinetobacter baumannii
Genomics
Insertion Sequences
Virulence Factors
Pre-antibiotic Era
Environmental Reservoirs
Acceso en línea:http://hdl.handle.net/2133/20524
http://hdl.handle.net/2133/20524
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20524
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Acinetobacter baumannii
Genomics
Insertion Sequences
Virulence Factors
Pre-antibiotic Era
Environmental Reservoirs
spellingShingle Acinetobacter baumannii
Genomics
Insertion Sequences
Virulence Factors
Pre-antibiotic Era
Environmental Reservoirs
Repizo, Guillermo Daniel
Espariz, Martín
Seravalle, Joana L.
Díaz Miloslavich, Juan Ignacio
Steimbrüch, Bruno A.
Shuman, Howard A.
Viale, Alejandro M.
Acinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms
topic_facet Acinetobacter baumannii
Genomics
Insertion Sequences
Virulence Factors
Pre-antibiotic Era
Environmental Reservoirs
description Acinetobacter baumannii represents nowadays an important nosocomial pathogen of poorly defined reservoirs outside the clinical setting. Here, we con ducted whole-genome sequencing analysis of the Acinetobacter sp. NCIMB8209 col lection strain, isolated in 1943 from the aerobic degradation (retting) of desert gu ayule shrubs. Strain NCIMB8209 contained a 3.75-Mb chromosome and a plasmid of 134 kb. Phylogenetic analysis based on core genes indicated NCIMB8209 affiliation to A. baumannii, a result supported by the identification of a chromosomal blaOXA 51-like gene. Seven genomic islands lacking antimicrobial resistance determinants, 5 regions encompassing phage-related genes, and notably, 93 insertion sequences (IS) were found in this genome. NCIMB8209 harbors most genes linked to persistence and virulence described in contemporary A. baumannii clinical strains, but many of the genes encoding components of surface structures are interrupted by IS. More over, defense genetic islands against biological aggressors such as type 6 secretion systems or CRISPR-cas are absent from this genome. These findings correlate with a low capacity of NCIMB8209 to form biofilm and pellicle, low motility on semisolid medium, and low virulence toward Galleria mellonella and Caenorhabditis elegans. Searching for catabolic genes and concomitant metabolic assays revealed the ability of NCIMB8209 to grow on a wide range of substances produced by plants, including aromatic acids and defense compounds against external aggressors. All the above features strongly suggest that NCIMB8209 has evolved specific adaptive features to a particular environmental niche. Moreover, they also revealed that the remarkable genetic plasticity identified in contemporary A. baumannii clinical strains represents an intrinsic characteristic of the species.
format publishedVersion
author Repizo, Guillermo Daniel
Espariz, Martín
Seravalle, Joana L.
Díaz Miloslavich, Juan Ignacio
Steimbrüch, Bruno A.
Shuman, Howard A.
Viale, Alejandro M.
author_facet Repizo, Guillermo Daniel
Espariz, Martín
Seravalle, Joana L.
Díaz Miloslavich, Juan Ignacio
Steimbrüch, Bruno A.
Shuman, Howard A.
Viale, Alejandro M.
author_sort Repizo, Guillermo Daniel
title Acinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms
title_short Acinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms
title_full Acinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms
title_fullStr Acinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms
title_full_unstemmed Acinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms
title_sort acinetobacter baumannii ncimb8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms
publisher American Society for Microbiology
publishDate 2021
url http://hdl.handle.net/2133/20524
http://hdl.handle.net/2133/20524
work_keys_str_mv AT repizoguillermodaniel acinetobacterbaumanniincimb8209arareenvironmentalstraindisplayingextensiveinsertionsequencemediatedgenomeremodelingresultinginthelossofexposedcellstructuresanddefensivemechanisms
AT esparizmartin acinetobacterbaumanniincimb8209arareenvironmentalstraindisplayingextensiveinsertionsequencemediatedgenomeremodelingresultinginthelossofexposedcellstructuresanddefensivemechanisms
AT seravallejoanal acinetobacterbaumanniincimb8209arareenvironmentalstraindisplayingextensiveinsertionsequencemediatedgenomeremodelingresultinginthelossofexposedcellstructuresanddefensivemechanisms
AT diazmiloslavichjuanignacio acinetobacterbaumanniincimb8209arareenvironmentalstraindisplayingextensiveinsertionsequencemediatedgenomeremodelingresultinginthelossofexposedcellstructuresanddefensivemechanisms
AT steimbruchbrunoa acinetobacterbaumanniincimb8209arareenvironmentalstraindisplayingextensiveinsertionsequencemediatedgenomeremodelingresultinginthelossofexposedcellstructuresanddefensivemechanisms
AT shumanhowarda acinetobacterbaumanniincimb8209arareenvironmentalstraindisplayingextensiveinsertionsequencemediatedgenomeremodelingresultinginthelossofexposedcellstructuresanddefensivemechanisms
AT vialealejandrom acinetobacterbaumanniincimb8209arareenvironmentalstraindisplayingextensiveinsertionsequencemediatedgenomeremodelingresultinginthelossofexposedcellstructuresanddefensivemechanisms
bdutipo_str Repositorios
_version_ 1764820411057438720

Ejemplares similares