Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii

Acinetobacter baumannii is nowadays a relevant nosocomial pathogen characterized by multidrug resistance (MDR) and concomitant difficulties to treat infections. OmpA is the most abundant A. baumannii outer membrane (OM) protein, and is involved in virulence, host-cell recognition, biofilm formatio...

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Autores principales: Viale, Alejandro M., Evans, Benjamin A.
Formato: publishedVersion
Lenguaje:Inglés
Publicado: Microbiology Society 2021
Materias:
Acinetobacter baumannii
Protein Evolution
OmpA
Recombination
Acceso en línea:http://hdl.handle.net/2133/20517
http://hdl.handle.net/2133/20517
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20517
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Acinetobacter baumannii
Protein Evolution
OmpA
Recombination
spellingShingle Acinetobacter baumannii
Protein Evolution
OmpA
Recombination
Viale, Alejandro M.
Evans, Benjamin A.
Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii
topic_facet Acinetobacter baumannii
Protein Evolution
OmpA
Recombination
description Acinetobacter baumannii is nowadays a relevant nosocomial pathogen characterized by multidrug resistance (MDR) and concomitant difficulties to treat infections. OmpA is the most abundant A. baumannii outer membrane (OM) protein, and is involved in virulence, host-cell recognition, biofilm formation, regulation of OM stability, permeability and antibiotic resistance. OmpA members are two‐domain proteins with an N‐terminal eight‐stranded β‐barrel domain with four external loops (ELs) interact ing with the environment, and a C‐terminal periplasmic domain binding non‐covalently to the peptidoglycan. Here, we combined data from genome sequencing, phylogenetic and multilocus sequence analyses from 975 strains/isolates of the Acinetobacter calcoaceticus/Acinetobacter baumannii complex (ACB), 946 from A. baumannii, to explore ompA microevolutionary divergence. Five major ompA variant groups were identified (V1 to V5) in A. baumannii, encompassing 52 different alleles coding for 23 different proteins. Polymorphisms were concentrated in five regions corresponding to the four ELs and the C‐terminal end, and provided evidence for intra‐genic recombination. ompA variants were not randomly distributed across the A. baumannii phylogeny, with the most frequent V1(lct)a1 allele found in most clonal complex 2 (CC2) strains and the second most frequent V2(lct)a1 allele in the majority of CC1 strains. Evidence was found for assortative exchanges of ompA alleles not only between separate A. baumannii lineages, but also different ACB species. The overall results have implications for A. baumannii evolution, epidemiology, virulence and vaccine design.
format publishedVersion
author Viale, Alejandro M.
Evans, Benjamin A.
author_facet Viale, Alejandro M.
Evans, Benjamin A.
author_sort Viale, Alejandro M.
title Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii
title_short Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii
title_full Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii
title_fullStr Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii
title_full_unstemmed Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii
title_sort microevolution in the major outer membrane protein ompa of acinetobacter baumannii
publisher Microbiology Society
publishDate 2021
url http://hdl.handle.net/2133/20517
http://hdl.handle.net/2133/20517
work_keys_str_mv AT vialealejandrom microevolutioninthemajoroutermembraneproteinompaofacinetobacterbaumannii
AT evansbenjamina microevolutioninthemajoroutermembraneproteinompaofacinetobacterbaumannii
bdutipo_str Repositorios
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