KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide

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Ralstonia solanacearum is the causative agent of bacterial wilt disease on a wide range of plant species. Besides the numerous bacterial activities required for host invasion, those involved in the adaptation to the plant environment are key for the success of infection. R. solanacearum ability to c...

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Autores principales: Tondo, María Laura, De Pedro-Jové, Roger, Vandecaveye, Agustina, Piskulic, Laura, Orellano, Elena G., Valls, Marc
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Frontiers Media 2021
Materias:
Ralstonia solanacearum
Bacterial Wilt
Oxidative Burst
KatE Catalase
Host Adaptation
Acceso en línea:http://hdl.handle.net/2133/20494
http://hdl.handle.net/2133/20494
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20494
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Ralstonia solanacearum
Bacterial Wilt
Oxidative Burst
KatE Catalase
Host Adaptation
spellingShingle Ralstonia solanacearum
Bacterial Wilt
Oxidative Burst
KatE Catalase
Host Adaptation
Tondo, María Laura
De Pedro-Jové, Roger
Vandecaveye, Agustina
Piskulic, Laura
Orellano, Elena G.
Valls, Marc
KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
topic_facet Ralstonia solanacearum
Bacterial Wilt
Oxidative Burst
KatE Catalase
Host Adaptation
description Ralstonia solanacearum is the causative agent of bacterial wilt disease on a wide range of plant species. Besides the numerous bacterial activities required for host invasion, those involved in the adaptation to the plant environment are key for the success of infection. R. solanacearum ability to cope with the oxidative burst produced by the plant is likely one of the activities required to grow parasitically. Among the multiple reactive oxygen species (ROS)-scavenging enzymes predicted in the R. solanacearum GMI1000 genome, a single monofunctional catalase (KatE) and two KatG bifunctional catalases were identified. In this work, we show that these catalase activities are active in bacterial protein extracts and demonstrate by gene disruption and mutant complementation that the monofunctional catalase activity is encoded by katE. Different strategies were used to evaluate the role of KatE in bacterial physiology and during the infection process that causes bacterial wilt. We show that the activity of the enzyme is maximal during exponential growth in vitro and this growth-phase regulation occurs at the transcriptional level. Our studies also demonstrate that katE expression is transcriptionally activated by HrpG, a central regulator of R. solanacearum induced upon contact with the plant cells. In addition, we reveal that even though both KatE and KatG catalase activities are induced upon hydrogen peroxide treatment, KatE has a major effect on bacterial survival under oxidative stress conditions and especially in the adaptive response of R. solanacearum to this oxidant. The katE mutant strain also exhibited differences in the structural characteristics of the biofilms developed on an abiotic surface in comparison to wild-type cells, but not in the overall amount of biofilm production. The role of catalase KatE during the interaction with its host plant tomato is also studied, revealing that disruption of this gene has no effect on R. solanacearum virulence or bacterial growth in leave tissues, which suggests a minor role for this catalase in bacterial fitness in planta. Our work provides the first characterization of the R. solanacearum catalases and identifies KatE as a bona fide monofunctional catalase with an important role in bacterial protection against oxidative stress.
format article
artículo
publishedVersion
author Tondo, María Laura
De Pedro-Jové, Roger
Vandecaveye, Agustina
Piskulic, Laura
Orellano, Elena G.
Valls, Marc
author_facet Tondo, María Laura
De Pedro-Jové, Roger
Vandecaveye, Agustina
Piskulic, Laura
Orellano, Elena G.
Valls, Marc
author_sort Tondo, María Laura
title KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_short KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_full KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_fullStr KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_full_unstemmed KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_sort kate from the bacterial plant pathogen ralstonia solanacearum is a monofunctional catalase controlled by hrpg that plays a major role in bacterial survival to hydrogen peroxide
publisher Frontiers Media
publishDate 2021
url http://hdl.handle.net/2133/20494
http://hdl.handle.net/2133/20494
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