First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná River

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Valid fish species identification is essential for biodiversity conservation and fisheries management. Here, we provide a sequence reference library based on mitochondrial cytochrome c oxidase subunit I for a valid identification of 79 freshwater fish species from the Lower Paraná River. Neighbour-...

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Autores principales: Díaz, Juan, Villanova, Gabriela Vanina, Brancolini, Florencia, Del Pazo, Felipe, Posner, Victoria María, Grimberg, Alexis, Arranz, Silvia Eda
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Public Library of Science (PLOS) 2021
Materias:
DNA Barcoding
Electron Transport Complex I
Paraná River
Fishes
Acceso en línea:http://hdl.handle.net/2133/20243
http://hdl.handle.net/2133/20243
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20243
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic DNA Barcoding
Electron Transport Complex I
Paraná River
Fishes
spellingShingle DNA Barcoding
Electron Transport Complex I
Paraná River
Fishes
Díaz, Juan
Villanova, Gabriela Vanina
Brancolini, Florencia
Del Pazo, Felipe
Posner, Victoria María
Grimberg, Alexis
Arranz, Silvia Eda
First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná River
topic_facet DNA Barcoding
Electron Transport Complex I
Paraná River
Fishes
description Valid fish species identification is essential for biodiversity conservation and fisheries management. Here, we provide a sequence reference library based on mitochondrial cytochrome c oxidase subunit I for a valid identification of 79 freshwater fish species from the Lower Paraná River. Neighbour-joining analysis based on K2P genetic distances formed non-overlapping clusters for almost all species with a 99% bootstrap support each. Identification was successful for 97.8% of species as the minimum genetic distance to the nearest neighbour exceeded the maximum intraspecific distance in all these cases. A barcoding gap of 2.5% was apparent for the whole data set with the exception of four cases. Within species distances ranged from 0.00% to 7.59%, while interspecific distances varied between 4.06% and 19.98%, without considering Odontesthes species with a minimum genetic distance of 0%. Sequence library validation was performed by applying BOLDs BIN analysis tool, Poisson Tree Processes model and Automatic Barcode Gap Discovery, along with a reliable taxonomic assignment by experts. Exhaustive revision of vouchers was performed when a conflicting assignment was detected after sequence analysis and BIN discordance evaluation. Thus, the sequence library presented here can be confidently used as a benchmark for identification of half of the fish species recorded for the Lower Paraná River.
format article
artículo
publishedVersion
author Díaz, Juan
Villanova, Gabriela Vanina
Brancolini, Florencia
Del Pazo, Felipe
Posner, Victoria María
Grimberg, Alexis
Arranz, Silvia Eda
author_facet Díaz, Juan
Villanova, Gabriela Vanina
Brancolini, Florencia
Del Pazo, Felipe
Posner, Victoria María
Grimberg, Alexis
Arranz, Silvia Eda
author_sort Díaz, Juan
title First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná River
title_short First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná River
title_full First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná River
title_fullStr First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná River
title_full_unstemmed First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná River
title_sort first dna barcode reference library for the identification of south american freshwater fish from the lower paraná river
publisher Public Library of Science (PLOS)
publishDate 2021
url http://hdl.handle.net/2133/20243
http://hdl.handle.net/2133/20243
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