Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs

The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven fa...

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Detalles Bibliográficos
Autores principales: Mastrochirico-Filho, Vito Antonio, Del Pazo, Felipe, Hata, Milene Elissa, Villanova, Gabriela Vanina, Foresti, Fausto, Vera, Manuel, Martínez, Paulino, Porto-Foresti, Fábio, Hashimoto, Diogo Teruo
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: MDPI 2021
Materias:
Aquaculture
Genetic Variability
Inbreeding
Breeding Programs
Acceso en línea:http://hdl.handle.net/2133/20241
http://hdl.handle.net/2133/20241
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20241
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Aquaculture
Genetic Variability
Inbreeding
Breeding Programs
spellingShingle Aquaculture
Genetic Variability
Inbreeding
Breeding Programs
Mastrochirico-Filho, Vito Antonio
Del Pazo, Felipe
Hata, Milene Elissa
Villanova, Gabriela Vanina
Foresti, Fausto
Vera, Manuel
Martínez, Paulino
Porto-Foresti, Fábio
Hashimoto, Diogo Teruo
Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs
topic_facet Aquaculture
Genetic Variability
Inbreeding
Breeding Programs
description The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks.
format article
artículo
publishedVersion
author Mastrochirico-Filho, Vito Antonio
Del Pazo, Felipe
Hata, Milene Elissa
Villanova, Gabriela Vanina
Foresti, Fausto
Vera, Manuel
Martínez, Paulino
Porto-Foresti, Fábio
Hashimoto, Diogo Teruo
author_facet Mastrochirico-Filho, Vito Antonio
Del Pazo, Felipe
Hata, Milene Elissa
Villanova, Gabriela Vanina
Foresti, Fausto
Vera, Manuel
Martínez, Paulino
Porto-Foresti, Fábio
Hashimoto, Diogo Teruo
author_sort Mastrochirico-Filho, Vito Antonio
title Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs
title_short Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs
title_full Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs
title_fullStr Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs
title_full_unstemmed Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs
title_sort assessing genetic diversity for a pre-breeding program in piaractus mesopotamicus by snps and ssrs
publisher MDPI
publishDate 2021
url http://hdl.handle.net/2133/20241
http://hdl.handle.net/2133/20241
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