Differential role of the T6SS in Acinetobacter baumannii virulence

Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in ho...

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Detalles Bibliográficos
Autores principales: Repizo, Guillermo Daniel, Gagné, Stéphanie, Foucault-Grunenwald, Marie-Laure, Borges, Vitor, Charpentier, Xavier, Limansky, Adriana S., Gomes, João Paulo, Viale, Alejandro M., Salcedo, Suzana P.
Formato: publishedVersion
Lenguaje:Inglés
Publicado: Public Library of Science (PLOS) 2021
Materias:
Acinetobacter baumannii
Virulence
T6SS
Acceso en línea:http://hdl.handle.net/2133/20230
http://hdl.handle.net/2133/20230
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20230
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Acinetobacter baumannii
Virulence
T6SS
spellingShingle Acinetobacter baumannii
Virulence
T6SS
Repizo, Guillermo Daniel
Gagné, Stéphanie
Foucault-Grunenwald, Marie-Laure
Borges, Vitor
Charpentier, Xavier
Limansky, Adriana S.
Gomes, João Paulo
Viale, Alejandro M.
Salcedo, Suzana P.
Differential role of the T6SS in Acinetobacter baumannii virulence
topic_facet Acinetobacter baumannii
Virulence
T6SS
description Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), reveal ing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addi tion, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.
format publishedVersion
author Repizo, Guillermo Daniel
Gagné, Stéphanie
Foucault-Grunenwald, Marie-Laure
Borges, Vitor
Charpentier, Xavier
Limansky, Adriana S.
Gomes, João Paulo
Viale, Alejandro M.
Salcedo, Suzana P.
author_facet Repizo, Guillermo Daniel
Gagné, Stéphanie
Foucault-Grunenwald, Marie-Laure
Borges, Vitor
Charpentier, Xavier
Limansky, Adriana S.
Gomes, João Paulo
Viale, Alejandro M.
Salcedo, Suzana P.
author_sort Repizo, Guillermo Daniel
title Differential role of the T6SS in Acinetobacter baumannii virulence
title_short Differential role of the T6SS in Acinetobacter baumannii virulence
title_full Differential role of the T6SS in Acinetobacter baumannii virulence
title_fullStr Differential role of the T6SS in Acinetobacter baumannii virulence
title_full_unstemmed Differential role of the T6SS in Acinetobacter baumannii virulence
title_sort differential role of the t6ss in acinetobacter baumannii virulence
publisher Public Library of Science (PLOS)
publishDate 2021
url http://hdl.handle.net/2133/20230
http://hdl.handle.net/2133/20230
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