The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis

Citrus canker is an important disease of citrus, whose causal agent is the bacterium Xanthomonas citri ssp. citri (Xcc). In previous studies, we found a group of Xcc mutants, generated by the insertion of the Tn5 transposon, which showed impaired ability to attach to an abiotic substrate. One...

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Detalles Bibliográficos
Autores principales: Conforte, Valeria P., Malamud, Florencia, Yaryura, Pablo M., Toum Terrones, Laila, Torres, Pablo S., De Pino, Verónica, Chazarreta, Cristian N., Gudesblat, Gustavo E., Castagnaro, Atilio P., Marano, María Rosa, Vojnov, Adrián A.
Formato: publishedVersion
Lenguaje:Inglés
Publicado: Wiley Open Access 2021
Materias:
Biofilms
Citrus
Flagella
Histone-Like Protein
Virulence
Xanthomonas
Acceso en línea:http://hdl.handle.net/2133/20156
http://hdl.handle.net/2133/20156
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20156
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Biofilms
Citrus
Flagella
Histone-Like Protein
Virulence
Xanthomonas
spellingShingle Biofilms
Citrus
Flagella
Histone-Like Protein
Virulence
Xanthomonas
Conforte, Valeria P.
Malamud, Florencia
Yaryura, Pablo M.
Toum Terrones, Laila
Torres, Pablo S.
De Pino, Verónica
Chazarreta, Cristian N.
Gudesblat, Gustavo E.
Castagnaro, Atilio P.
Marano, María Rosa
Vojnov, Adrián A.
The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis
topic_facet Biofilms
Citrus
Flagella
Histone-Like Protein
Virulence
Xanthomonas
description Citrus canker is an important disease of citrus, whose causal agent is the bacterium Xanthomonas citri ssp. citri (Xcc). In previous studies, we found a group of Xcc mutants, generated by the insertion of the Tn5 transposon, which showed impaired ability to attach to an abiotic substrate. One of these mutants carries the Tn5 insertion in hupB, a gene encoding a bacterial histone-like protein, homologue to the β-subunit of the Heat-Unstable (HU) nucleoid protein of Escherichia coli. These types of protein are necessary to maintain the bacterial nucleoid organization and the global regulation of gene expression. Here, we characterized the influence of the mutation in hupB regarding Xcc biofilm forma tion and virulence. The mutant strain hupB was incapable of swimming in soft agar, whereas its complemented strain partially recovered this phenotype. Electron microscope imaging revealed that impaired motility of hupB was a consequence of the absence of the flagellum. Comparison of the expression of flagellar genes between the wild-type strain and hupB showed that the mutant exhibited decreased expression of fliC (encoding flagellin). The hupB mutant also displayed reduced virulence compared with the wild-type strain when they were used to infect Citrus lemon plants using different infection methods. Our results therefore show that the histone-like protein HupB plays an essential role in the pathogenesis of Xcc through the regulation of biofilm formation and biosynthesis of the flagellum.
format publishedVersion
author Conforte, Valeria P.
Malamud, Florencia
Yaryura, Pablo M.
Toum Terrones, Laila
Torres, Pablo S.
De Pino, Verónica
Chazarreta, Cristian N.
Gudesblat, Gustavo E.
Castagnaro, Atilio P.
Marano, María Rosa
Vojnov, Adrián A.
author_facet Conforte, Valeria P.
Malamud, Florencia
Yaryura, Pablo M.
Toum Terrones, Laila
Torres, Pablo S.
De Pino, Verónica
Chazarreta, Cristian N.
Gudesblat, Gustavo E.
Castagnaro, Atilio P.
Marano, María Rosa
Vojnov, Adrián A.
author_sort Conforte, Valeria P.
title The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis
title_short The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis
title_full The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis
title_fullStr The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis
title_full_unstemmed The histone-like protein HupB influences biofilm formation and virulence in Xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis
title_sort histone-like protein hupb influences biofilm formation and virulence in xanthomonas citri ssp. citri through the regulation of flagellar biosynthesis
publisher Wiley Open Access
publishDate 2021
url http://hdl.handle.net/2133/20156
http://hdl.handle.net/2133/20156
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