PthA4AT, a 7.5-repeats transcription activator-like (TAL) effector from Xanthomonas citri ssp. citri, triggers citrus canker resistance

Transcription activator-like effectors (TALEs) are important effec tors of Xanthomonas spp. that manipulate the transcriptome of the host plant, conferring susceptibility or resistance to bacterial infection. Xanthomonas citri ssp. citri variant AT (X. citri AT ) triggers a host-specific h...

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Detalles Bibliográficos
Autores principales: Roeschlin, Roxana Andrea, Uviedo, Facundo, García, Lucila, Molina, María Celeste, Favaro, María Alejandra, Chiesa, María Amalia, Tasselli, Sabrina, Franco-Zorrilla, José Manuel, Forment, Javier, Gadea, José, Marano, María Rosa
Formato: publishedVersion
Lenguaje:Inglés
Publicado: Wiley Open Access 2021
Materias:
Citrus
Hypersensitive Response
Nicotiana benthamiana
Transcription Activator-Like (TAL) Effectors
Xanthomonas citri
Acceso en línea:http://hdl.handle.net/2133/20129
http://hdl.handle.net/2133/20129
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20129
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Citrus
Hypersensitive Response
Nicotiana benthamiana
Transcription Activator-Like (TAL) Effectors
Xanthomonas citri
spellingShingle Citrus
Hypersensitive Response
Nicotiana benthamiana
Transcription Activator-Like (TAL) Effectors
Xanthomonas citri
Roeschlin, Roxana Andrea
Uviedo, Facundo
García, Lucila
Molina, María Celeste
Favaro, María Alejandra
Chiesa, María Amalia
Tasselli, Sabrina
Franco-Zorrilla, José Manuel
Forment, Javier
Gadea, José
Marano, María Rosa
PthA4AT, a 7.5-repeats transcription activator-like (TAL) effector from Xanthomonas citri ssp. citri, triggers citrus canker resistance
topic_facet Citrus
Hypersensitive Response
Nicotiana benthamiana
Transcription Activator-Like (TAL) Effectors
Xanthomonas citri
description Transcription activator-like effectors (TALEs) are important effec tors of Xanthomonas spp. that manipulate the transcriptome of the host plant, conferring susceptibility or resistance to bacterial infection. Xanthomonas citri ssp. citri variant AT (X. citri AT ) triggers a host-specific hypersensitive response (HR) that suppresses citrus canker development. However, the bacterial effector that elicits this process is unknown. In this study, we show that a 7.5-repeat TALE is responsible for triggering the HR. PthA4AT was identified within the pthA repertoire of X. citri AT followed by assay of the effects on different hosts. The mode of action of PthA4AT was characterized using protein-binding microarrays and testing the effects of dele tion of the nuclear localization signals and activation domain on plant responses. PthA4AT is able to bind DNA and activate transcrip tion in an effector binding element-dependent manner. Moreover, HR requires PthA4AT nuclear localization, suggesting the activation of executor resistance (R) genes in host and non-host plants. This is the first case where a TALE of unusually short length performs a biological function by means of its repeat domain, indicating that the action of these effectors to reprogramme the host transcriptome following nuclear localization is not limited to ‘classical’ TALEs.
format publishedVersion
author Roeschlin, Roxana Andrea
Uviedo, Facundo
García, Lucila
Molina, María Celeste
Favaro, María Alejandra
Chiesa, María Amalia
Tasselli, Sabrina
Franco-Zorrilla, José Manuel
Forment, Javier
Gadea, José
Marano, María Rosa
author_facet Roeschlin, Roxana Andrea
Uviedo, Facundo
García, Lucila
Molina, María Celeste
Favaro, María Alejandra
Chiesa, María Amalia
Tasselli, Sabrina
Franco-Zorrilla, José Manuel
Forment, Javier
Gadea, José
Marano, María Rosa
author_sort Roeschlin, Roxana Andrea
title PthA4AT, a 7.5-repeats transcription activator-like (TAL) effector from Xanthomonas citri ssp. citri, triggers citrus canker resistance
title_short PthA4AT, a 7.5-repeats transcription activator-like (TAL) effector from Xanthomonas citri ssp. citri, triggers citrus canker resistance
title_full PthA4AT, a 7.5-repeats transcription activator-like (TAL) effector from Xanthomonas citri ssp. citri, triggers citrus canker resistance
title_fullStr PthA4AT, a 7.5-repeats transcription activator-like (TAL) effector from Xanthomonas citri ssp. citri, triggers citrus canker resistance
title_full_unstemmed PthA4AT, a 7.5-repeats transcription activator-like (TAL) effector from Xanthomonas citri ssp. citri, triggers citrus canker resistance
title_sort ptha4at, a 7.5-repeats transcription activator-like (tal) effector from xanthomonas citri ssp. citri, triggers citrus canker resistance
publisher Wiley Open Access
publishDate 2021
url http://hdl.handle.net/2133/20129
http://hdl.handle.net/2133/20129
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