Transmembrane prolines mediate signal sensing and decoding in Bacillus subtilis DesK histidine kinase

Environmental awareness is an essential attribute of all organisms. The homeoviscous adaptation system of Bacillus subtilis provides a powerful experimen tal model for the investigation of stimulus detection and signaling mechanisms at the molecular level. These bacteria sense the order of membran...

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Detalles Bibliográficos
Autores principales: Fernández, Pilar, Porrini, Lucía, Albanesi, Daniela, Abriata, Luciano A., Dal Peraro, Matteo, De Mendoza, Diego, Mansilla, María Cecilia
Formato: publishedVersion
Lenguaje:Inglés
Publicado: American Society for Microbiology 2021
Materias:
Histidine Kinase
Proline
Thermosensing
Two-component Regulatory Systems
Acceso en línea:http://hdl.handle.net/2133/20127
http://hdl.handle.net/2133/20127
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20127
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Histidine Kinase
Proline
Thermosensing
Two-component Regulatory Systems
spellingShingle Histidine Kinase
Proline
Thermosensing
Two-component Regulatory Systems
Fernández, Pilar
Porrini, Lucía
Albanesi, Daniela
Abriata, Luciano A.
Dal Peraro, Matteo
De Mendoza, Diego
Mansilla, María Cecilia
Transmembrane prolines mediate signal sensing and decoding in Bacillus subtilis DesK histidine kinase
topic_facet Histidine Kinase
Proline
Thermosensing
Two-component Regulatory Systems
description Environmental awareness is an essential attribute of all organisms. The homeoviscous adaptation system of Bacillus subtilis provides a powerful experimen tal model for the investigation of stimulus detection and signaling mechanisms at the molecular level. These bacteria sense the order of membrane lipids with the transmembrane (TM) protein DesK, which has an N-terminal sensor domain and an intracellular catalytic effector domain. DesK exhibits autokinase activity as well as phosphotransferase and phosphatase activities toward a cognate response regulator, DesR, that controls the expression of an enzyme that remodels membrane fluidity when the temperature drops below 30°C. Membrane fluidity signals are transmit ted from the DesK sensor domain to the effector domain via rotational movements of a connecting 2-helix coiled coil (2-HCC). Previous molecular dynamic simulations suggested important roles for TM prolines in transducing the initial signals of mem brane fluidity status to the 2-HCC. Here, we report that individual replacement of prolines in DesKs TM1 and TM5 helices by alanine (DesKPA) locked DesK in a phosphatase-ON state, abrogating membrane fluidity responses. An unbiased muta genic screen identified the L174P replacement in the internal side of the repeated heptad of the 2-HCC structure that alleviated the signaling defects of every trans membrane DesKPA substitution. Moreover, substitutions by proline in other internal positions of the 2-HCC reestablished the kinase-ON state of the DesKPA mutants. These results imply that TM prolines are essential for finely tuned signal generation by the N-terminal sensor helices, facilitating a conformational control by the meta stable 2-HCC domain of the DesK signaling state.
format publishedVersion
author Fernández, Pilar
Porrini, Lucía
Albanesi, Daniela
Abriata, Luciano A.
Dal Peraro, Matteo
De Mendoza, Diego
Mansilla, María Cecilia
author_facet Fernández, Pilar
Porrini, Lucía
Albanesi, Daniela
Abriata, Luciano A.
Dal Peraro, Matteo
De Mendoza, Diego
Mansilla, María Cecilia
author_sort Fernández, Pilar
title Transmembrane prolines mediate signal sensing and decoding in Bacillus subtilis DesK histidine kinase
title_short Transmembrane prolines mediate signal sensing and decoding in Bacillus subtilis DesK histidine kinase
title_full Transmembrane prolines mediate signal sensing and decoding in Bacillus subtilis DesK histidine kinase
title_fullStr Transmembrane prolines mediate signal sensing and decoding in Bacillus subtilis DesK histidine kinase
title_full_unstemmed Transmembrane prolines mediate signal sensing and decoding in Bacillus subtilis DesK histidine kinase
title_sort transmembrane prolines mediate signal sensing and decoding in bacillus subtilis desk histidine kinase
publisher American Society for Microbiology
publishDate 2021
url http://hdl.handle.net/2133/20127
http://hdl.handle.net/2133/20127
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