Site-specific recombination at XerC/D sites mediates the formation and resolution of plasmid co-integrates carrying a blaOXA-58- and TnaphA6-resistance module in Acinetobacter baumannii
Members of the genus Acinetobacter possess distinct plasmid types which provide effective platforms for the acquisition, evolution, and dissemination of antimicrobial resistance structures. Many plasmid-borne resistance structures are bordered by short DNA sequences providing potential recognitio...
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Formato: | publishedVersion |
Lenguaje: | Inglés |
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Frontiers Media
2021
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Acceso en línea: | http://hdl.handle.net/2133/20110 http://hdl.handle.net/2133/20110 |
Aporte de: |
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I15-R121-2133-20110 |
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record_format |
dspace |
institution |
Universidad Nacional de Rosario |
institution_str |
I-15 |
repository_str |
R-121 |
collection |
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) |
language |
Inglés |
orig_language_str_mv |
eng |
topic |
Acinetobacter baumannii blaOXA-58 Carbapenems Resistance XerC/D Site-specific Recombination Antimicrobial Resistance Plasmids |
spellingShingle |
Acinetobacter baumannii blaOXA-58 Carbapenems Resistance XerC/D Site-specific Recombination Antimicrobial Resistance Plasmids Cameranesi, Marcela Morán-Barrio, Jorgelina Limansky, Adriana S. Repizo, Guillermo Daniel Viale, Alejandro M. Site-specific recombination at XerC/D sites mediates the formation and resolution of plasmid co-integrates carrying a blaOXA-58- and TnaphA6-resistance module in Acinetobacter baumannii |
topic_facet |
Acinetobacter baumannii blaOXA-58 Carbapenems Resistance XerC/D Site-specific Recombination Antimicrobial Resistance Plasmids |
description |
Members of the genus Acinetobacter possess distinct plasmid types which provide
effective platforms for the acquisition, evolution, and dissemination of antimicrobial
resistance structures. Many plasmid-borne resistance structures are bordered by
short DNA sequences providing potential recognition sites for the host XerC and
XerD site-specific tyrosine recombinases (XerC/D-like sites). However, whether these
sites are active in recombination and how they assist the mobilization of associated
resistance structures is still poorly understood. Here we characterized the plasmids
carried by Acinetobacter baumannii Ab242, a multidrug-resistant clinical strain belonging
to the ST104 (Oxford scheme) which produces an OXA-58 carbapenem-hydrolyzing
class-D β-lactamase (CHDL). Plasmid sequencing and characterization of replication,
stability, and adaptive modules revealed the presence in Ab242 of three novel plasmids
lacking self-transferability functions which were designated pAb242_9, pAb242_12,
and pAb242_25, respectively. Among them, only pAb242_25 was found to carry an
adaptive module encompassing an ISAba825-blaOXA-58 arrangement accompanied
by a TnaphA6 transposon, the whole structure conferring simultaneous resistance to
carbapenems and aminoglycosides. Ab242 plasmids harbor several XerC/D-like sites,
with most sites found in pAb242_25 located in the vicinity or within the adaptive
module described above. Electrotransformation of susceptible A. nosocomialis cells
with Ab242 plasmids followed by imipenem selection indicated that the transforming
plasmid form was a co-integrate resulting from the fusion of pAb242_25 and pAb242_12.
Further characterization by cloning and sequencing studies indicated that a XerC/D
site in pAb242_25 and another in pAb242_12 provided the active sister pair for the
inter-molecular site-specific recombination reaction mediating the fusion of these two
plasmids. Moreover, the resulting co-integrate was found also to undergo intra-molecular
resolution at the new pair of XerC/D sites generated during fusion thus regenerating
the original pAb242_25 and pAb242_12 plasmids. These observations provide the irst evidence indicating that XerC/D-like sites in A. baumannii plasmids can provide
active pairs for site-specific recombination mediating inter-molecular fusions and intra molecular resolutions. The overall results shed light on the evolutionary dynamics of
A. baumannii plasmids and the underlying mechanisms of dissemination of genetic
structures responsible for carbapenem and other antibiotics resistance among the
Acinetobacter clinical population. |
format |
publishedVersion |
author |
Cameranesi, Marcela Morán-Barrio, Jorgelina Limansky, Adriana S. Repizo, Guillermo Daniel Viale, Alejandro M. |
author_facet |
Cameranesi, Marcela Morán-Barrio, Jorgelina Limansky, Adriana S. Repizo, Guillermo Daniel Viale, Alejandro M. |
author_sort |
Cameranesi, Marcela |
title |
Site-specific recombination at XerC/D sites mediates the formation and resolution of plasmid co-integrates carrying a blaOXA-58- and TnaphA6-resistance module in Acinetobacter baumannii |
title_short |
Site-specific recombination at XerC/D sites mediates the formation and resolution of plasmid co-integrates carrying a blaOXA-58- and TnaphA6-resistance module in Acinetobacter baumannii |
title_full |
Site-specific recombination at XerC/D sites mediates the formation and resolution of plasmid co-integrates carrying a blaOXA-58- and TnaphA6-resistance module in Acinetobacter baumannii |
title_fullStr |
Site-specific recombination at XerC/D sites mediates the formation and resolution of plasmid co-integrates carrying a blaOXA-58- and TnaphA6-resistance module in Acinetobacter baumannii |
title_full_unstemmed |
Site-specific recombination at XerC/D sites mediates the formation and resolution of plasmid co-integrates carrying a blaOXA-58- and TnaphA6-resistance module in Acinetobacter baumannii |
title_sort |
site-specific recombination at xerc/d sites mediates the formation and resolution of plasmid co-integrates carrying a blaoxa-58- and tnapha6-resistance module in acinetobacter baumannii |
publisher |
Frontiers Media |
publishDate |
2021 |
url |
http://hdl.handle.net/2133/20110 http://hdl.handle.net/2133/20110 |
work_keys_str_mv |
AT cameranesimarcela sitespecificrecombinationatxercdsitesmediatestheformationandresolutionofplasmidcointegratescarryingablaoxa58andtnapha6resistancemoduleinacinetobacterbaumannii AT moranbarriojorgelina sitespecificrecombinationatxercdsitesmediatestheformationandresolutionofplasmidcointegratescarryingablaoxa58andtnapha6resistancemoduleinacinetobacterbaumannii AT limanskyadrianas sitespecificrecombinationatxercdsitesmediatestheformationandresolutionofplasmidcointegratescarryingablaoxa58andtnapha6resistancemoduleinacinetobacterbaumannii AT repizoguillermodaniel sitespecificrecombinationatxercdsitesmediatestheformationandresolutionofplasmidcointegratescarryingablaoxa58andtnapha6resistancemoduleinacinetobacterbaumannii AT vialealejandrom sitespecificrecombinationatxercdsitesmediatestheformationandresolutionofplasmidcointegratescarryingablaoxa58andtnapha6resistancemoduleinacinetobacterbaumannii |
bdutipo_str |
Repositorios |
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1764820411300708352 |