Regulatory T cells but not NKT I cells are modulated by a single low-dose cyclophosphamide in a B cell lymphoma tumor-model

Aim: Experimental and clinical studies showed that the administration of cyclophosphamide (Cy) in low doses leads to an enhancement of the antitumor immune response. Our objective was to study the modulation, if any, by low dose Cy, of T regulatory (Treg) and natural killer T (NKT) I cells, two cell...

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Autores principales: Rico, María José, Rozados, Viviana R., Mainetti, Leandro Ernesto, Zacarías Fluck, Mariano, Matar, Pablo, Scharovsky, O. Graciela
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: MORION LLC 2012
Materias:
T regulatory cells
NKT I cells
cyclophosphamide
lymphoma
Acceso en línea:http://hdl.handle.net/2133/2006
http://hdl.handle.net/2133/2006
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-2006
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv en
topic T regulatory cells
NKT I cells
cyclophosphamide
lymphoma
spellingShingle T regulatory cells
NKT I cells
cyclophosphamide
lymphoma
Rico, María José
Rozados, Viviana R.
Mainetti, Leandro Ernesto
Zacarías Fluck, Mariano
Matar, Pablo
Scharovsky, O. Graciela
Regulatory T cells but not NKT I cells are modulated by a single low-dose cyclophosphamide in a B cell lymphoma tumor-model
topic_facet T regulatory cells
NKT I cells
cyclophosphamide
lymphoma
description Aim: Experimental and clinical studies showed that the administration of cyclophosphamide (Cy) in low doses leads to an enhancement of the antitumor immune response. Our objective was to study the modulation, if any, by low dose Cy, of T regulatory (Treg) and natural killer T (NKT) I cells, two cell populations of the innate immune response with opposing effects on the tumors, in a rat B cell lymphoma model. Methods: Inbred e rats were challenged s.c. with L-TACB lymphoma and on day 14 animals were distributed in two groups. Treated: injected i.p. with cyclophosphamide (10mg/kg of body weight) and Control: injected i.p. with saline. Blood samples were taken from days 0 to 21 and the percentage of T regulatory and natural killer T I cells were determined by flow cytometry. Results: We found that the increase of natural and inducible T regulatory cells of peripheral blood achieved during tumor growth was significantly downregulated by cyclophosphamide. On the contrary, natural killer T I cells were not modulated by the treatment. Conclusion: The antimetastatic effect of a single low dose of Cy would be due, at least in part, to downregulation of natural and inducible T regulatory cells.
format article
artículo
publishedVersion
author Rico, María José
Rozados, Viviana R.
Mainetti, Leandro Ernesto
Zacarías Fluck, Mariano
Matar, Pablo
Scharovsky, O. Graciela
author_facet Rico, María José
Rozados, Viviana R.
Mainetti, Leandro Ernesto
Zacarías Fluck, Mariano
Matar, Pablo
Scharovsky, O. Graciela
author_sort Rico, María José
title Regulatory T cells but not NKT I cells are modulated by a single low-dose cyclophosphamide in a B cell lymphoma tumor-model
title_short Regulatory T cells but not NKT I cells are modulated by a single low-dose cyclophosphamide in a B cell lymphoma tumor-model
title_full Regulatory T cells but not NKT I cells are modulated by a single low-dose cyclophosphamide in a B cell lymphoma tumor-model
title_fullStr Regulatory T cells but not NKT I cells are modulated by a single low-dose cyclophosphamide in a B cell lymphoma tumor-model
title_full_unstemmed Regulatory T cells but not NKT I cells are modulated by a single low-dose cyclophosphamide in a B cell lymphoma tumor-model
title_sort regulatory t cells but not nkt i cells are modulated by a single low-dose cyclophosphamide in a b cell lymphoma tumor-model
publisher MORION LLC
publishDate 2012
url http://hdl.handle.net/2133/2006
http://hdl.handle.net/2133/2006
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bdutipo_str Repositorios
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