A comparison of methods for the detection of Ascochyta rabiei in chickpea seeds

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Seed health is one of the most important factors affecting the quality of chickpea (Cicer arietinum) seeds. The present study aimed to compare and identify the best incubation methods for detecting Ascochyta rabiei associated with chickpea seeds. Four protocols were compared for their sensiti...

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Autores principales: Sautua, Francisco José, Casey, Santiago Agustín, Zapata, Raúl Lorenzo, Scandiani, María Mercedes, Carmona, Marcelo Aníbal
Formato: publishedVersion
Lenguaje:Inglés
Publicado: Grupo Paulista de Fitopatologia 2021
Materias:
Ascochyta blight
Chickpea
Cicer
Standard Blotter Test
PDA Plate Test
Acceso en línea:http://hdl.handle.net/2133/20038
http://hdl.handle.net/2133/20038
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-20038
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Ascochyta blight
Chickpea
Cicer
Standard Blotter Test
PDA Plate Test
spellingShingle Ascochyta blight
Chickpea
Cicer
Standard Blotter Test
PDA Plate Test
Sautua, Francisco José
Casey, Santiago Agustín
Zapata, Raúl Lorenzo
Scandiani, María Mercedes
Carmona, Marcelo Aníbal
A comparison of methods for the detection of Ascochyta rabiei in chickpea seeds
topic_facet Ascochyta blight
Chickpea
Cicer
Standard Blotter Test
PDA Plate Test
description Seed health is one of the most important factors affecting the quality of chickpea (Cicer arietinum) seeds. The present study aimed to compare and identify the best incubation methods for detecting Ascochyta rabiei associated with chickpea seeds. Four protocols were compared for their sensitivity in detecting A. rabiei: T1) Incubation on paper substrate or filter paper method (blotter test) without surface disinfection, T2) Blotter test through the water restriction technique, T3) PDA plate test, and T4) MEA plate test. Four independent chickpea seed lots, naturally infected with A. rabiei, were sampled from Córdoba Province and other four were sampled from Buenos Aires Province, Argentina. Each treatment was applied to a total of 400 seeds from each locality for the methods to be comparable. T2 and T3 were statistically more sensitive in detecting A. rabiei-infected seeds from Córdoba. Only these two treatments were repeated for seeds from Buenos Aires Province, and T3 proved to be more sensitive; thus, it is recommended for routine sanitary analysis of chickpea seeds.
format publishedVersion
author Sautua, Francisco José
Casey, Santiago Agustín
Zapata, Raúl Lorenzo
Scandiani, María Mercedes
Carmona, Marcelo Aníbal
author_facet Sautua, Francisco José
Casey, Santiago Agustín
Zapata, Raúl Lorenzo
Scandiani, María Mercedes
Carmona, Marcelo Aníbal
author_sort Sautua, Francisco José
title A comparison of methods for the detection of Ascochyta rabiei in chickpea seeds
title_short A comparison of methods for the detection of Ascochyta rabiei in chickpea seeds
title_full A comparison of methods for the detection of Ascochyta rabiei in chickpea seeds
title_fullStr A comparison of methods for the detection of Ascochyta rabiei in chickpea seeds
title_full_unstemmed A comparison of methods for the detection of Ascochyta rabiei in chickpea seeds
title_sort comparison of methods for the detection of ascochyta rabiei in chickpea seeds
publisher Grupo Paulista de Fitopatologia
publishDate 2021
url http://hdl.handle.net/2133/20038
http://hdl.handle.net/2133/20038
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