P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation

P1 is a R2R3-MYB transcription factor that regulates the accumulation of a specific group of flavonoids in maize floral tissues, such as flavones and phlobaphenes. P1 is also highly expressed in leaves of maize landraces adapted to high altitudes and higher levels of UV-B radiation. In this work,...

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Autores principales: Rius, Sebastián P., Emiliani, Julia, Casati, Paula
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Frontiers Media 2021
Materias:
Epigenetic Regulation
Flavonoids
P1 Transcription Factor
Zea Mays
UV-B
Acceso en línea:http://hdl.handle.net/2133/19988
http://hdl.handle.net/2133/19988
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19988
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Epigenetic Regulation
Flavonoids
P1 Transcription Factor
Zea Mays
UV-B
spellingShingle Epigenetic Regulation
Flavonoids
P1 Transcription Factor
Zea Mays
UV-B
Rius, Sebastián P.
Emiliani, Julia
Casati, Paula
P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation
topic_facet Epigenetic Regulation
Flavonoids
P1 Transcription Factor
Zea Mays
UV-B
description P1 is a R2R3-MYB transcription factor that regulates the accumulation of a specific group of flavonoids in maize floral tissues, such as flavones and phlobaphenes. P1 is also highly expressed in leaves of maize landraces adapted to high altitudes and higher levels of UV-B radiation. In this work, we analyzed the epigenetic regulation of the P1 gene by UV-B in leaves of different maize landraces. Our results demonstrate that DNA methylation in the P1 proximal promoter, intron1 and intron2 is decreased by UV-B in all lines analyzed; however, the basal DNA methylation levels are lower in the landraces than in B73, a low altitude inbred line. DNA demethylation by UV-B is accompanied by a decrease in H3 methylation at Lys 9 and 27, and by an increase in H3 acetylation. smRNAs complementary to specific regions of the proximal promoter and of intron 2 3 ′ end are also decreased by UV-B; interestingly, P1 smRNA levels are lower in the landraces than in B73 both under control conditions and after UV-B exposure, suggesting that smRNAs regulate P1 expression by UV-B in maize leaves. Finally, we investigated if different P1 targets in flower tissues are also regulated by this transcription factor in response to UV-B. Some targets analyzed show an induction in maize landraces in response to UV-B, with higher basal expression levels in the landraces than in B73; however, not all the transcripts analyzed were found to be regulated by UV-B in leaves.
format article
artículo
publishedVersion
author Rius, Sebastián P.
Emiliani, Julia
Casati, Paula
author_facet Rius, Sebastián P.
Emiliani, Julia
Casati, Paula
author_sort Rius, Sebastián P.
title P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation
title_short P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation
title_full P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation
title_fullStr P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation
title_full_unstemmed P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation
title_sort p1 epigenetic regulation in leaves of high altitude maize landraces: effect of uv-b radiation
publisher Frontiers Media
publishDate 2021
url http://hdl.handle.net/2133/19988
http://hdl.handle.net/2133/19988
work_keys_str_mv AT riussebastianp p1epigeneticregulationinleavesofhighaltitudemaizelandraceseffectofuvbradiation
AT emilianijulia p1epigeneticregulationinleavesofhighaltitudemaizelandraceseffectofuvbradiation
AT casatipaula p1epigeneticregulationinleavesofhighaltitudemaizelandraceseffectofuvbradiation
bdutipo_str Repositorios
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