Renal expression and urinary excretion of Na-K-2Cl cotransporter in obstructive nephropathy

Renal damage due to urinary tract obstruction accounts for up to 30% of acute kidney injury in paediatrics and adults. Bilateral ureteral obstruction (BUO) is associated with polyuria and reduced urinary concentrating capacity. We investigated the renal handling of water and electrolytes together...

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Detalles Bibliográficos
Autores principales: Brandoni, Anabel, Torres, Adriana Mónica
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Hindawi 2021
Materias:
Obstructive Nephropathy
Na-K-2Cl Cotransporter
Urinary Excretion
Renal Expression
Kidney Injury
Biomarker
Renal Function Recovery
Acceso en línea:http://hdl.handle.net/2133/19909
http://hdl.handle.net/2133/19909
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19909
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Obstructive Nephropathy
Na-K-2Cl Cotransporter
Urinary Excretion
Renal Expression
Kidney Injury
Biomarker
Renal Function Recovery
spellingShingle Obstructive Nephropathy
Na-K-2Cl Cotransporter
Urinary Excretion
Renal Expression
Kidney Injury
Biomarker
Renal Function Recovery
Brandoni, Anabel
Torres, Adriana Mónica
Renal expression and urinary excretion of Na-K-2Cl cotransporter in obstructive nephropathy
topic_facet Obstructive Nephropathy
Na-K-2Cl Cotransporter
Urinary Excretion
Renal Expression
Kidney Injury
Biomarker
Renal Function Recovery
description Renal damage due to urinary tract obstruction accounts for up to 30% of acute kidney injury in paediatrics and adults. Bilateral ureteral obstruction (BUO) is associated with polyuria and reduced urinary concentrating capacity. We investigated the renal handling of water and electrolytes together with the renal expression and the urinary excretion of the Na-K-Cl cotransporter (NKCC2) after 1 (BUO-1), 2 (BUO-2), and 7 (BUO-7) days of release of BUO. Immunoblotting and immunohistochemical studies showed that NKCC2 expression was upregulated in apical membranes both from BUO-2 and from BUO-7 rats. The apical membrane expression, where NKCC2 is functional, may be sufficient to normalize water, potassium, sodium, and osmolytes tubular handling. NKCC2 abundance in homogenates and mRNA levels of NKCC2 was significantly decreased in almost all groups suggesting a decrease in the synthesis of the transporter. Urinary excretion of NKCC2 was increased in BUO-7 groups. These data suggest that the upregulation in the expression of NKCC2 in apical membranes during the postobstructive phase of BUO could contribute to improving the excretion of sodium and consequently also the excretion of potassium, osmolytes, and water. Moreover, the increase in urinary excretion of NKCC2 in BUO-7 group could be a potential additional biomarker of renal function recovery
format article
artículo
publishedVersion
author Brandoni, Anabel
Torres, Adriana Mónica
author_facet Brandoni, Anabel
Torres, Adriana Mónica
author_sort Brandoni, Anabel
title Renal expression and urinary excretion of Na-K-2Cl cotransporter in obstructive nephropathy
title_short Renal expression and urinary excretion of Na-K-2Cl cotransporter in obstructive nephropathy
title_full Renal expression and urinary excretion of Na-K-2Cl cotransporter in obstructive nephropathy
title_fullStr Renal expression and urinary excretion of Na-K-2Cl cotransporter in obstructive nephropathy
title_full_unstemmed Renal expression and urinary excretion of Na-K-2Cl cotransporter in obstructive nephropathy
title_sort renal expression and urinary excretion of na-k-2cl cotransporter in obstructive nephropathy
publisher Hindawi
publishDate 2021
url http://hdl.handle.net/2133/19909
http://hdl.handle.net/2133/19909
work_keys_str_mv AT brandonianabel renalexpressionandurinaryexcretionofnak2clcotransporterinobstructivenephropathy
AT torresadrianamonica renalexpressionandurinaryexcretionofnak2clcotransporterinobstructivenephropathy
bdutipo_str Repositorios
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