Suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressed tobacco plants

Crop yield reduction due to salinity is a growing agronomical concern in many regions. Increased production of reactive oxygen species (ROS) in plant cells accompanies many abiotic stresses including salinity, acting as toxic and signaling molecules during plant stress responses. While ROS are ge...

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Autores principales: Lodeyro, Anabella F., Giró, Mariana, Poli, Hugo O., Bettucci, Gabriel, Cortadi, Adriana, Ferri, Alejandro M., Carrillo, Néstor
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Public Library of Science (PLOS) 2021
Materias:
Crop Yield
Reactive Oxygen Species
Chloroplasts
Salt Stress
Tobacco
Acceso en línea:http://hdl.handle.net/2133/19677
http://hdl.handle.net/2133/19677
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19677
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Crop Yield
Reactive Oxygen Species
Chloroplasts
Salt Stress
Tobacco
spellingShingle Crop Yield
Reactive Oxygen Species
Chloroplasts
Salt Stress
Tobacco
Lodeyro, Anabella F.
Giró, Mariana
Poli, Hugo O.
Bettucci, Gabriel
Cortadi, Adriana
Ferri, Alejandro M.
Carrillo, Néstor
Suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressed tobacco plants
topic_facet Crop Yield
Reactive Oxygen Species
Chloroplasts
Salt Stress
Tobacco
description Crop yield reduction due to salinity is a growing agronomical concern in many regions. Increased production of reactive oxygen species (ROS) in plant cells accompanies many abiotic stresses including salinity, acting as toxic and signaling molecules during plant stress responses. While ROS are generated in various cellular compartments, chloroplasts represent a main source in the light, and plastid ROS synthesis and/or elimination have been manipulated to improve stress tolerance. Transgenic tobacco plants expressing a plastid-targeted cyanobacterial flavodoxin, a flavoprotein that prevents ROS accumulation specifically in chloroplasts, displayed increased tolerance to many environmental stresses, including drought, excess irradiation, extreme temperatures and iron starvation. Surprisingly, flavodoxin expression failed to protect transgenic plants against NaCl toxicity. However, when high salt was directly applied to leaf discs, flavodoxin did increase tolerance, as reflected by preservation of chlorophylls, carotenoids and photosynthetic activities. Flavodoxin decreased salt-dependent ROS accumulation in leaf tissue from discs and whole plants, but this decline did not improve tolerance at the whole plant level. NaCl accumulation in roots, as well as increased osmotic pressure and salt-induced root damage, were not prevented by flavodoxin expression. The results indicate that ROS formed in chloroplasts have a marginal effect on plant responses during salt stress, and that sensitive targets are present in roots which are not protected by flavodoxin.
format article
artículo
publishedVersion
author Lodeyro, Anabella F.
Giró, Mariana
Poli, Hugo O.
Bettucci, Gabriel
Cortadi, Adriana
Ferri, Alejandro M.
Carrillo, Néstor
author_facet Lodeyro, Anabella F.
Giró, Mariana
Poli, Hugo O.
Bettucci, Gabriel
Cortadi, Adriana
Ferri, Alejandro M.
Carrillo, Néstor
author_sort Lodeyro, Anabella F.
title Suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressed tobacco plants
title_short Suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressed tobacco plants
title_full Suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressed tobacco plants
title_fullStr Suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressed tobacco plants
title_full_unstemmed Suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressed tobacco plants
title_sort suppression of reactive oxygen species accumulation in chloroplasts prevents leaf damage but not growth arrest in salt-stressed tobacco plants
publisher Public Library of Science (PLOS)
publishDate 2021
url http://hdl.handle.net/2133/19677
http://hdl.handle.net/2133/19677
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bdutipo_str Repositorios
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