TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells
Cell penetrating peptides, also known as protein transduction domains, have the capacity to ubiquitously cross cellular membranes carrying many different cargos with negligible cytotoxicity. As a result, they have emerged as a powerful tool for macromolecular deliverybased therapies. In this study,...
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Formato: | article artículo publishedVersion |
Lenguaje: | Inglés |
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Public Library of Science (PLOS)
2021
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Acceso en línea: | http://hdl.handle.net/2133/19675 http://hdl.handle.net/2133/19675 |
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I15-R121-2133-19675 |
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institution |
Universidad Nacional de Rosario |
institution_str |
I-15 |
repository_str |
R-121 |
collection |
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) |
language |
Inglés |
topic |
TAT Regulatory Protein Bacteria Redox Proteins Cytoprotection Neurons Oxidative Stress |
spellingShingle |
TAT Regulatory Protein Bacteria Redox Proteins Cytoprotection Neurons Oxidative Stress Balaban, Cecilia Lucía Banchio, Claudia Ceccarelli, Eduardo Augusto TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells |
topic_facet |
TAT Regulatory Protein Bacteria Redox Proteins Cytoprotection Neurons Oxidative Stress |
description |
Cell penetrating peptides, also known as protein transduction domains, have the capacity to
ubiquitously cross cellular membranes carrying many different cargos with negligible cytotoxicity. As a result, they have emerged as a powerful tool for macromolecular deliverybased therapies. In this study, catalytically active bacterial Ferredoxin-NADP+ reductase
(LepFNR) and Heme oxygenase (LepHO) fused to the HIV TAT-derived protein transduction peptide (TAT) were efficiently transduced to neuroblastoma SHSY-5Y cells. Proteins
entered the cells through an endocytic pathway showing a time/concentration dependent
mechanism that was clearly modulated by the nature of the cargo protein. Since ferredoxinNADP+ reductases and heme oxygenases have been implicated in mechanisms of oxidative
stress defense, neuroblastoma cells simultaneously transduced with TAT-LepFNR and
TAT-LepHO were challenged by H2O2 incubations to judge the cytoprotective power of
these bacterial enzymes. Accumulation of reactive oxygen species was significantly
reduced in these transduced neuronal cells. Moreover, measurements of metabolic viability,
membrane integrity, and cell survival indicated that these cells showed a better tolerance to
oxidative stress. Our results open the possibility for the application of transducible active
redox proteins to overcome the damage elicited by oxidative stress in cells and tissues. |
format |
article artículo publishedVersion |
author |
Balaban, Cecilia Lucía Banchio, Claudia Ceccarelli, Eduardo Augusto |
author_facet |
Balaban, Cecilia Lucía Banchio, Claudia Ceccarelli, Eduardo Augusto |
author_sort |
Balaban, Cecilia Lucía |
title |
TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells |
title_short |
TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells |
title_full |
TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells |
title_fullStr |
TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells |
title_full_unstemmed |
TAT-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells |
title_sort |
tat-mediated transduction of bacterial redox proteins generates a cytoprotective effect on neuronal cells |
publisher |
Public Library of Science (PLOS) |
publishDate |
2021 |
url |
http://hdl.handle.net/2133/19675 http://hdl.handle.net/2133/19675 |
work_keys_str_mv |
AT balabancecilialucia tatmediatedtransductionofbacterialredoxproteinsgeneratesacytoprotectiveeffectonneuronalcells AT banchioclaudia tatmediatedtransductionofbacterialredoxproteinsgeneratesacytoprotectiveeffectonneuronalcells AT ceccarellieduardoaugusto tatmediatedtransductionofbacterialredoxproteinsgeneratesacytoprotectiveeffectonneuronalcells |
bdutipo_str |
Repositorios |
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