CNBP controls transcription by unfolding DNA G-quadruplex structures

Guanine-rich DNA strands can fold into noncanonical four-stranded secondary structures named G-quadruplexes (G4). Experimental evidences suggest that G4-DNA surrounding transcription start sites act as cis-regulatory elements by either stimulating or inhibiting gene transcription. Therefore, prot...

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Autores principales:	David, Aldana P., Pipier, Angélique, Pascutti, Federico, Binolfi, Andrés, Weiner, Andrea María Julia, Challier, Emilse, Heckel, Sofía, Calsou, Patrick, Gomez, Dennis, Calcaterra, Nora B., Armas, Pablo
Formato:	article artículo publishedVersion
Lenguaje:	Inglés
Publicado:	Oxford University Press 2021
Materias:	Cellular Nucleic Acid-Binding Protein (CNBP) Transcription Factors DNA G-quadruplex Structures Guanine
Acceso en línea:	http://hdl.handle.net/2133/19667 http://hdl.handle.net/2133/19667
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-19667
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	Cellular Nucleic Acid-Binding Protein (CNBP) Transcription Factors DNA G-quadruplex Structures Guanine
spellingShingle	Cellular Nucleic Acid-Binding Protein (CNBP) Transcription Factors DNA G-quadruplex Structures Guanine David, Aldana P. Pipier, Angélique Pascutti, Federico Binolfi, Andrés Weiner, Andrea María Julia Challier, Emilse Heckel, Sofía Calsou, Patrick Gomez, Dennis Calcaterra, Nora B. Armas, Pablo CNBP controls transcription by unfolding DNA G-quadruplex structures
topic_facet	Cellular Nucleic Acid-Binding Protein (CNBP) Transcription Factors DNA G-quadruplex Structures Guanine
description	Guanine-rich DNA strands can fold into noncanonical four-stranded secondary structures named G-quadruplexes (G4). Experimental evidences suggest that G4-DNA surrounding transcription start sites act as cis-regulatory elements by either stimulating or inhibiting gene transcription. Therefore, proteins able to target and regulate specific G4 formation/unfolding are crucial for G4- mediated transcriptional control. Here we present data revealing that CNBP acts in vitro as a G4- unfolding protein over a tetramolecular G4 formed by the TG4T oligonucleotide, as well as over the G4 folded in the promoters of several oncogenes. CNBP depletion in cellulo led to a reduction in the transcription of endogenous KRAS, suggesting a regulatory role of CNBP in relieving the transcriptional abrogation due to G4 formation. CNBP activity was also assayed over the evolutionary conserved G4 enhancing the transcription of NOGGIN (NOG) developmental gene. CNBP unfolded in vitro NOG G4 and experiments performed in cellulo and in vivo in developing zebrafish showed a repressive role of CNBP on the transcription of this gene by G4 unwinding. Our results shed light on the mechanisms underlying CNBP way of action, as well as reinforce the notion about the existence and function of G4s in whole living organisms
format	article artículo publishedVersion
author	David, Aldana P. Pipier, Angélique Pascutti, Federico Binolfi, Andrés Weiner, Andrea María Julia Challier, Emilse Heckel, Sofía Calsou, Patrick Gomez, Dennis Calcaterra, Nora B. Armas, Pablo
author_facet	David, Aldana P. Pipier, Angélique Pascutti, Federico Binolfi, Andrés Weiner, Andrea María Julia Challier, Emilse Heckel, Sofía Calsou, Patrick Gomez, Dennis Calcaterra, Nora B. Armas, Pablo
author_sort	David, Aldana P.
title	CNBP controls transcription by unfolding DNA G-quadruplex structures
title_short	CNBP controls transcription by unfolding DNA G-quadruplex structures
title_full	CNBP controls transcription by unfolding DNA G-quadruplex structures
title_fullStr	CNBP controls transcription by unfolding DNA G-quadruplex structures
title_full_unstemmed	CNBP controls transcription by unfolding DNA G-quadruplex structures
title_sort	cnbp controls transcription by unfolding dna g-quadruplex structures
publisher	Oxford University Press
publishDate	2021
url	http://hdl.handle.net/2133/19667 http://hdl.handle.net/2133/19667
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bdutipo_str	Repositorios
_version_	1764820411109867521

CNBP controls transcription by unfolding DNA G-quadruplex structures

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