Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection

Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay ba...

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Autores principales:	Ferreira, Virginia, Pianzzola, María J., Vilaró, Francisco L., Galván, Guillermo A., Tondo, María Laura, Rodriguez, María V., Orellano, Elena G., Valls, Marc
Formato:	article artículo publishedVersion
Lenguaje:	Inglés
Publicado:	Frontiers Media 2021
Materias:	Bacterial Wilt Ralstonia solanacearum Solanum commersonii Potato Plant Breeding Disease Resistance Latent Infections
Acceso en línea:	http://hdl.handle.net/2133/19659 http://hdl.handle.net/2133/19659
Aporte de:	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario

id	I15-R121-2133-19659
record_format	dspace
institution	Universidad Nacional de Rosario
institution_str	I-15
repository_str	R-121
collection	Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language	Inglés
orig_language_str_mv	eng
topic	Bacterial Wilt Ralstonia solanacearum Solanum commersonii Potato Plant Breeding Disease Resistance Latent Infections
spellingShingle	Bacterial Wilt Ralstonia solanacearum Solanum commersonii Potato Plant Breeding Disease Resistance Latent Infections Ferreira, Virginia Pianzzola, María J. Vilaró, Francisco L. Galván, Guillermo A. Tondo, María Laura Rodriguez, María V. Orellano, Elena G. Valls, Marc Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection
topic_facet	Bacterial Wilt Ralstonia solanacearum Solanum commersonii Potato Plant Breeding Disease Resistance Latent Infections
description	Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.
format	article artículo publishedVersion
author	Ferreira, Virginia Pianzzola, María J. Vilaró, Francisco L. Galván, Guillermo A. Tondo, María Laura Rodriguez, María V. Orellano, Elena G. Valls, Marc
author_facet	Ferreira, Virginia Pianzzola, María J. Vilaró, Francisco L. Galván, Guillermo A. Tondo, María Laura Rodriguez, María V. Orellano, Elena G. Valls, Marc
author_sort	Ferreira, Virginia
title	Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection
title_short	Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection
title_full	Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection
title_fullStr	Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection
title_full_unstemmed	Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection
title_sort	interspecific potato breeding lines display differential colonization patterns and induced defense responses after ralstonia solanacearum infection
publisher	Frontiers Media
publishDate	2021
url	http://hdl.handle.net/2133/19659 http://hdl.handle.net/2133/19659
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bdutipo_str	Repositorios
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Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection

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