Endocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves

Proper cholesterol transport is crucial for the functionality of cells. In C. elegans, certain cholesterol derivatives called dafachronic acids (DAs) govern the entry into diapause. In their absence, worms form a developmentally arrested dauer larva. Thus, cholesterol transport to appropriate plac...

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Autores principales: Galles, Celina, Prez, Gastón, Penkov, Sider, Boland, Sebastian, Porta, Exequiel Oscar Jesús, Altabe, Silvia Graciela, Labadie, Guillermo Roberto, Schmidt, Ulrike, Knölker, Hans-Joachim, Kurzchalia, Teymuras V., De Mendoza, Diego
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Nature 2021
Materias:
Endocannabinoids
Caenorhabditis elegans
Cholesterol Transport
Glycosphingolipids
Acceso en línea:http://hdl.handle.net/2133/19648
http://hdl.handle.net/2133/19648
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19648
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
topic Endocannabinoids
Caenorhabditis elegans
Cholesterol Transport
Glycosphingolipids
spellingShingle Endocannabinoids
Caenorhabditis elegans
Cholesterol Transport
Glycosphingolipids
Galles, Celina
Prez, Gastón
Penkov, Sider
Boland, Sebastian
Porta, Exequiel Oscar Jesús
Altabe, Silvia Graciela
Labadie, Guillermo Roberto
Schmidt, Ulrike
Knölker, Hans-Joachim
Kurzchalia, Teymuras V.
De Mendoza, Diego
Endocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves
topic_facet Endocannabinoids
Caenorhabditis elegans
Cholesterol Transport
Glycosphingolipids
description Proper cholesterol transport is crucial for the functionality of cells. In C. elegans, certain cholesterol derivatives called dafachronic acids (DAs) govern the entry into diapause. In their absence, worms form a developmentally arrested dauer larva. Thus, cholesterol transport to appropriate places for DA biosynthesis warrants the reproductive growth. Recently, we discovered a novel class of glycosphingolipids, PEGCs, required for cholesterol mobilization/transport from internal storage pools. Here, we identify other components involved in this process. We found that strains lacking polyunsaturated fatty acids (PUFAs) undergo increased dauer arrest when grown without cholesterol. This correlates with the depletion of the PUFA-derived endocannabinoids 2-arachidonoyl glycerol and anandamide. Feeding of these endocannabinoids inhibits dauer formation caused by PUFAs defciency or impaired cholesterol trafcking (e.g. in Niemann-Pick C1 or DAF-7/TGF-β mutants). Moreover, in parallel to PEGCs, endocannabinoids abolish the arrest induced by cholesterol depletion. These fndings reveal an unsuspected function of endocannabinoids in cholesterol trafcking regulation.
format article
artículo
publishedVersion
author Galles, Celina
Prez, Gastón
Penkov, Sider
Boland, Sebastian
Porta, Exequiel Oscar Jesús
Altabe, Silvia Graciela
Labadie, Guillermo Roberto
Schmidt, Ulrike
Knölker, Hans-Joachim
Kurzchalia, Teymuras V.
De Mendoza, Diego
author_facet Galles, Celina
Prez, Gastón
Penkov, Sider
Boland, Sebastian
Porta, Exequiel Oscar Jesús
Altabe, Silvia Graciela
Labadie, Guillermo Roberto
Schmidt, Ulrike
Knölker, Hans-Joachim
Kurzchalia, Teymuras V.
De Mendoza, Diego
author_sort Galles, Celina
title Endocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves
title_short Endocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves
title_full Endocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves
title_fullStr Endocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves
title_full_unstemmed Endocannabinoids in Caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves
title_sort endocannabinoids in caenorhabditis elegans are essential for the mobilization of cholesterol from internal reserves
publisher Nature
publishDate 2021
url http://hdl.handle.net/2133/19648
http://hdl.handle.net/2133/19648
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