3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection

Citrus canker is a disease caused by the phytopathogen Xanthomonas citri subsp. citri (Xcc), bacterium which is unable to survive out of the host for extended periods of time. Once established inside the plant, the pathogen must compete for resources and evade the defenses of the host cell. Howeve...

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Detalles Bibliográficos
Autores principales: Tomassetti, Mauro, Garavaglia, Betiana S., Vranych, Cecilia V., Gottig, Natalia, Ottado, Jorgelina, Gramajo, Hugo Cesar, Diacovich, Lautaro
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Public Library of Science (PLOS) 2020
Materias:
Xanthomonas citri subsp. citri
Citrus Canker
Methylcrotonyl Coenzyme A (CoA) carboxylase complex
Acceso en línea:http://hdl.handle.net/2133/19538
http://hdl.handle.net/2133/19538
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19538
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Xanthomonas citri subsp. citri
Citrus Canker
Methylcrotonyl Coenzyme A (CoA) carboxylase complex
spellingShingle Xanthomonas citri subsp. citri
Citrus Canker
Methylcrotonyl Coenzyme A (CoA) carboxylase complex
Tomassetti, Mauro
Garavaglia, Betiana S.
Vranych, Cecilia V.
Gottig, Natalia
Ottado, Jorgelina
Gramajo, Hugo Cesar
Diacovich, Lautaro
3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection
topic_facet Xanthomonas citri subsp. citri
Citrus Canker
Methylcrotonyl Coenzyme A (CoA) carboxylase complex
description Citrus canker is a disease caused by the phytopathogen Xanthomonas citri subsp. citri (Xcc), bacterium which is unable to survive out of the host for extended periods of time. Once established inside the plant, the pathogen must compete for resources and evade the defenses of the host cell. However, a number of aspects of Xcc metabolic and nutritional state, during the epiphytic stage and at different phases of infection, are poorly characterized. The 3-methylcrotonyl-CoA carboxylase complex (MCC) is an essential enzyme for the catabolism of the branched-chain amino acid leucine, which prevents the accumulation of toxic intermediaries, facilitates the generation of branched chain fatty acids and/or provides energy to the cell. The MCC complexes belong to a group of acyl-CoA carboxylases (ACCase) enzymes dependent of biotin. In this work, we have identified two ORFs (XAC0263 and XAC0264) encoding for the α and β subunits of an acyl-CoA carboxylase complex from Xanthomonas and demonstrated that this enzyme has MCC activity both in vitro and in vivo. We also found that this MCC complex is conserved in a group of pathogenic gram negative bacteria. The generation and analysis of an Xcc mutant strain deficient in MCC showed less canker lesions in the interaction with the host plant, suggesting that the expression of these proteins is necessary for Xcc fitness during infection.
format article
artículo
publishedVersion
author Tomassetti, Mauro
Garavaglia, Betiana S.
Vranych, Cecilia V.
Gottig, Natalia
Ottado, Jorgelina
Gramajo, Hugo Cesar
Diacovich, Lautaro
author_facet Tomassetti, Mauro
Garavaglia, Betiana S.
Vranych, Cecilia V.
Gottig, Natalia
Ottado, Jorgelina
Gramajo, Hugo Cesar
Diacovich, Lautaro
author_sort Tomassetti, Mauro
title 3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection
title_short 3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection
title_full 3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection
title_fullStr 3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection
title_full_unstemmed 3-methylcrotonyl Coenzyme A (CoA) carboxylase complex is involved in the Xanthomonas citri subsp. citri lifestyle during citrus infection
title_sort 3-methylcrotonyl coenzyme a (coa) carboxylase complex is involved in the xanthomonas citri subsp. citri lifestyle during citrus infection
publisher Public Library of Science (PLOS)
publishDate 2020
url http://hdl.handle.net/2133/19538
http://hdl.handle.net/2133/19538
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