Simultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”

The simultaneous determination of adhesion and deformability parameters of erythrocytes was carried out through a microfluidic device, which uses an inverted optical microscope with new image acquisition and analysis technologies. Also, an update of the models describing erythrocyte adhesion and def...

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Autores principales: Londero, Carolina M., Riquelme, Bibiana Doris
Formato: article artículo publishedVersion
Lenguaje:Inglés
Publicado: Springer 2020
Materias:
Hemorheology
Microfluidic chamber
Red blood cell adhesion
Red blood cell deformation
Acceso en línea:http://hdl.handle.net/2133/19394
http://hdl.handle.net/2133/19394
Aporte de:
Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR) de Universidad Nacional de Rosario
id I15-R121-2133-19394
record_format dspace
institution Universidad Nacional de Rosario
institution_str I-15
repository_str R-121
collection Repositorio Hipermedial de la Universidad Nacional de Rosario (UNR)
language Inglés
orig_language_str_mv eng
topic Hemorheology
Microfluidic chamber
Red blood cell adhesion
Red blood cell deformation
spellingShingle Hemorheology
Microfluidic chamber
Red blood cell adhesion
Red blood cell deformation
Londero, Carolina M.
Riquelme, Bibiana Doris
Simultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”
topic_facet Hemorheology
Microfluidic chamber
Red blood cell adhesion
Red blood cell deformation
description The simultaneous determination of adhesion and deformability parameters of erythrocytes was carried out through a microfluidic device, which uses an inverted optical microscope with new image acquisition and analysis technologies. Also, an update of the models describing erythrocyte adhesion and deformation was proposed. Measurements were carried out with red blood cells suspended in saline solution with human serum albumin at different concentrations. Erythrocytes adhered to a glass surface were subjected to different low shear stress (from 0.04 to 0.25 Pa), causing cellular deformation and dissociation. The maximum value obtained of the erythrocyte deformability index was 0.3, and that of the adhesion energy per unit area was 1.1 × 10-6 Pa m, both according to previous works. The obtained images of RBCs adhered to glass reveal that the adhesion is stronger in a single point of the cell, suggesting a ligand migration that concentrates the adhesion in a "spike-like tip" in the cell. Moreover, adhesion energy results indicate that the energy required to separate erythrocytes in media with a lower albumin concentration is greater. Both results could be explained by the mobility of membrane receptors.
format article
artículo
publishedVersion
author Londero, Carolina M.
Riquelme, Bibiana Doris
author_facet Londero, Carolina M.
Riquelme, Bibiana Doris
author_sort Londero, Carolina M.
title Simultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”
title_short Simultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”
title_full Simultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”
title_fullStr Simultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”
title_full_unstemmed Simultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”
title_sort simultaneous determination of human erythrocyte deformability and adhesion energy: a novel approach using a microfluidic chamber and the “glass effect”
publisher Springer
publishDate 2020
url http://hdl.handle.net/2133/19394
http://hdl.handle.net/2133/19394
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bdutipo_str Repositorios
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